Genetic Dissection of a Genomic Region with Pleiotropic Effects on Domestication Traits in Maize Reveals Multiple Linked QTL

Lemmon, Z. H., Doebley, J. F. (September 2014) Genetic Dissection of a Genomic Region with Pleiotropic Effects on Domestication Traits in Maize Reveals Multiple Linked QTL. Genetics, 198 (1). pp. 345-53. ISSN 0016-6731

URL: http://www.ncbi.nlm.nih.gov/pubmed/24950893
DOI: 10.1534/genetics.114.165845

Abstract

The domesticated crop maize and its wild progenitor, teosinte, have been used in numerous experiments to investigate the nature of divergent morphologies. This study examines a poorly understood region on the fifth chromosome of maize associated with a number of traits under selection during domestication, using a quantitative trait locus (QTL) mapping population specific to the fifth chromosome. In contrast with other major domestication loci in maize where large-effect, highly pleiotropic, single genes are responsible for phenotypic effects, our study found the region on chromosome five fractionates into multiple-QTL regions, none with singularly large effects. The smallest 1.5-LOD support interval for a QTL contained 54 genes, one of which was a MADS MIKC(C) transcription factor, a family of proteins implicated in many developmental programs. We also used simulated trait data sets to investigate the power of our mapping population to identify QTL for which there is a single underlying causal gene. This analysis showed that while QTL for traits controlled by single genes can be accurately mapped, our population design can detect no more than approximately 4.5 QTL per trait even when there are 100 causal genes. Thus when a trait is controlled by >/=5 genes in the simulated data, the number of detected QTL can represent a simplification of the underlying causative factors. Our results show how a QTL region with effects on several domestication traits may be due to multiple linked QTL of small effect as opposed to a single gene with large and pleiotropic effects.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics
organism description > plant > maize
CSHL Authors:
Communities: CSHL labs > Lippman lab
Depositing User: Matt Covey
Date: September 2014
Date Deposited: 07 Nov 2014 16:49
Last Modified: 07 Nov 2014 16:49
PMCID: PMC4174946
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30896

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