Global Ablation of Mouse Rab11a Impairs Early Embryogenesis and Matrix Metalloproteinase Secretion

Yu, S., Yehia, G., Wang, J., Stypulkowski, E., Sakamori, R., Jiang, P., Hernandez-Enriquez, E. B., Tran, T. S., Bonder, E. M., Guo, W., Gao, N. (September 2014) Global Ablation of Mouse Rab11a Impairs Early Embryogenesis and Matrix Metalloproteinase Secretion. Journal of Biological Chemistry, 289 (46). pp. 32030-32043. ISSN 0021-9258

URL: http://www.ncbi.nlm.nih.gov/pubmed/25271168
DOI: 10.1074/jbc.M113.538223

Abstract

Rab11a has been conceived as a prominent regulatory component of the recycling endosome, which acts as a nexus in the endo- and exo-cytotic networks. The precise in vivo role of Rab11a in mouse embryonic development is unknown. We globally ablated Rab11a and examined the phenotypic and molecular outcomes in Rab11a-null blastocysts and mouse embryonic fibroblasts (MEFs). Using multiple trafficking assays and complementation analyses, we determined, among multiple important membrane-associated and soluble cargos, the critical contribution of Rab11a vesicular traffic to the secretion of multiple soluble MMPs. Rab11a-null embryos were able to properly form normal blastocysts but died at peri-implantation stages. Our data suggested that Rab11a critically controls mouse blastocyst development and soluble MMP secretion.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing
organism description > animal > developmental stage > embryo
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > matrix metalloproteinase
organism description > animal > mammal > rodent > mouse
CSHL Authors:
Communities: CSHL labs > Mills lab
CSHL Cancer Center Shared Resources > Gene Targeting Service
Depositing User: Matt Covey
Date: 30 September 2014
Date Deposited: 09 Oct 2014 19:06
Last Modified: 01 Dec 2015 21:26
PMCID: PMC4231680
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30832

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