Considerations when investigating IncRNA function in vivo

Bassett, A. R., Akhtar, A., Barlow, D. P., Bird, A. P., Brockdorff, N., Duboule, D., Ephrussi, A., Ferguson-Smith, A. C., Gingeras, T. R., Haerty, W., Higgs, D. R., Miska, E. A., Ponting, C. P. (2014) Considerations when investigating IncRNA function in vivo. Elife, 3. ISSN 2050-084X

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URL: http://www.ncbi.nlm.nih.gov/pubmed/25124674
DOI: 10.7554/eLife.03058

Abstract

Although a small number of the vast array of animal long non-coding RNAs (IncRNAs) have known effects on cellular processes examined in vitro, the extent of their contributions to normal cell processes throughout development, differentiation and disease for the most part remains less clear. Phenotypes arising from deletion of an entire genomic locus cannot be unequivocally attributed either to the loss of the IncRNA per se or to the associated loss of other overlapping DNA regulatory elements. The distinction between cis- or trans-effects is also often problematic. We discuss the advantages and challenges associated with the current techniques for studying the in vivo function of IncRNAs in the light of different models of IncRNA molecular mechanism, and reflect on the design of experiments to mutate IncRNA loci. These considerations should assist in the further investigation of these transcriptional products of the genome.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > long non-coding RNA
CSHL Authors:
Communities: CSHL labs > Gingeras lab
Depositing User: Matt Covey
Date Deposited: 03 Oct 2014 14:52
Last Modified: 03 Nov 2017 18:52
PMCID: PMC4132285
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30822

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