Respiratory nitrate reductase of Escherichia coli. Sequence identification of the large subunit gene

McPherson, M. J., Baron, A. J., Pappin, D. J., Wootton, J. C. (November 1984) Respiratory nitrate reductase of Escherichia coli. Sequence identification of the large subunit gene. FEBS Lett, 177 (2). pp. 260-4. ISSN 0014-5793 (Print)0014-5793 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/6094247
DOI: 10.1016/0014-5793(84)81295-8

Abstract

The nucleotide sequence is presented of part of the transcription initiation end of the nar operon of Escherichia coli K12, which encodes the respiratory nitrate reductase complex. The first coding sequence transcribed is the narG gene, encoding the large catalytic molybdoprotein of the complex. This sequence was assigned unambiguously by automated N-terminal amino acid sequencing of the purified large subunit. The deduced partial amino acid sequence of this polypeptide is hydrophilic and rich in basic residues. Membrane insertion does not involve N-terminal proteolytic processing of this subunit.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Base Sequence DNA Restriction Enzymes Escherichia coli/ enzymology/genetics Genes Genes, Bacterial Macromolecular Substances Nitrate Reductase Nitrate Reductases/ genetics/isolation & purification Oxygen Consumption Plasmids Species Specificity
Subjects: organism description > bacteria > escherichia coli
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
CSHL Authors:
Communities: CSHL labs > Pappin lab
Depositing User: Matt Covey
Date: 19 November 1984
Date Deposited: 10 Sep 2014 20:20
Last Modified: 10 Sep 2014 20:20
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30779

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