Gene sequence for the 9 kDa component of Photosystem II from the cyanobacterium Phormidium laminosum indicates similarities between cyanobacterial and other leader sequences

Wallace, T. P., Stewart, A. C., Pappin, D., Howe, C. J. (1989) Gene sequence for the 9 kDa component of Photosystem II from the cyanobacterium Phormidium laminosum indicates similarities between cyanobacterial and other leader sequences. Molecular & General Genetics, 216 (2-3). pp. 334-339. ISSN 00268925 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/2501648
DOI: 10.1007/bf00334373

Abstract

A 9 kDa polypeptide which is loosely attached to the inner surface of the thylakoid membrane and is important for the oxygen-evolving activity of Photosystem II in the thermophilic cyanobacterium Phormidium laminosum has been purified, a partial amino acid sequence obtained and its gene cloned and sequenced. The derived amino acid sequence indicates that the 9 kDa polypeptide is initially synthesised with an N-terminal leader sequence of 44 amino acids to direct it across the thylakoid membrane. The leader sequence consists of a positively charged N-terminal region, a long hydrophobic region and a typical cleavage site. These features have analogous counterparts in the "thylakoid-transfer domain" of lumenal polypeptides from chloroplasts of higher plants. These findings support the view of the proposed function of this domain in the two-stage processing model for import of lumenal, nuclear-encoded polypeptides. In addition, there is striking primary sequence homology between the leader sequences of the 9 kDa polypeptide and those of alkaline phosphatase (from the periplasmic space of Escherichia coli) and, particularly in the region of the cleavage site, the 16 kDa polypeptide of the oxygen-evolving apparatus in the thylakoid lumen of spinach chloroplasts. © 1989 Springer-Verlag.

Item Type: Paper
Uncontrolled Keywords: Cyanobacteria Evolution Leader sequence Photosystem II Thylakoid chlorophyll DNA signal peptide vegetable protein amino acid sequence article Cyanobacterium genetics molecular cloning molecular genetics nucleotide sequence photosynthesis sequence homology Base Sequence Cloning, Molecular Molecular Sequence Data Photosynthetic Reaction Center, Plant Plant Proteins Protein Sorting Signals Sequence Homology, Nucleic Acid Support, Non-U.S. Gov't
Subjects: organism description > bacteria
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > polypeptides
CSHL Authors:
Communities: CSHL labs > Pappin lab
Depositing User: Matt Covey
Date: 1989
Date Deposited: 18 Sep 2014 20:00
Last Modified: 18 Sep 2014 20:00
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30755

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