Coronavirus IBV glycopolypeptides: locational studies using proteases and saponin, a membrane permeabilizer

Cavanagh, D., Davis, P. J., Pappin, D. J. (February 1986) Coronavirus IBV glycopolypeptides: locational studies using proteases and saponin, a membrane permeabilizer. Virus Res, 4 (2). pp. 145-56. ISSN 0168-1702 (Print)0168-1702 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/3010596
DOI: 10.1016/0168-1702(86)90038-9

Abstract

[35S]methionine-labelled avian infectious bronchitis virus (IBV) (strain 41) and its purified protein components and virions of IBV-Beaudette were incubated with 10 proteases. Several proteases hydrolysed all or some of the membrane glycopolypeptide (M; Mr 30K) and removed about 1.3K of peptide from the amino-(N-)-terminus plus both glycans, as determined by SDS-polyacrylamide gel electrophoresis. N-terminal analysis of [3H]isoleucine-labelled M after hydrolysis by bromelain revealed that the first nine residues had been removed. After the virions had been permeabilised with saponin, a further 2.5K decrease in molecular weight was produced and this was shown to be from the carboxy-(C-)terminus. When considered with the hydropathicity plot analysis of the amino acid sequence of M (Boursnell, M.E.G. et al., 1984, Virus Res. 1, 303-313) these results suggest that as few as 9-20 N-terminal amino acid residues may protrude at the outer membrane surface and that there is a highly protease sensitive sequence of an estimated 20-25 residues at the C-terminus of M exposed in the lumen of the virion. S2 but not S1 was cleaved to a major glycopolypeptide of approximately 71K by several proteases, and to 76K by trypsin. N-terminal sequencing of the 71K glycopolypeptide revealed that it had the same N-terminus as intact S2. After hydrolysis in the presence and absence of saponin it was concluded that S2 is very sensitive to hydrolysis near its carboxy terminus at residues close to the outer membrane surface.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Coronaviridae/ analysis Electrophoresis, Polyacrylamide Gel Hydrolysis Infectious bronchitis virus/ analysis/ultrastructure Microscopy, Electron Peptide Hydrolases/metabolism Saponins Viral Envelope Proteins/ analysis Virion/metabolism
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > polypeptides
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > Protease
organism description > virus
CSHL Authors:
Communities: CSHL labs > Pappin lab
Depositing User: Matt Covey
Date: February 1986
Date Deposited: 18 Sep 2014 19:33
Last Modified: 18 Sep 2014 19:33
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30748

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