Cloning and sequencing of the gene encoding the spike protein of the coronavirus IBV

Binns, M. M., Boursnell, M. E., Cavanagh, D., Pappin, D. J., Brown, T. D. (April 1985) Cloning and sequencing of the gene encoding the spike protein of the coronavirus IBV. J Gen Virol, 66 ( P. pp. 719-26. ISSN 0022-1317 (Print)0022-1317 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/2984314
DOI: 10.1099/0022-1317-66-4-719

Abstract

RNA sequences encoding the surface projection (spike) of the coronavirus infectious bronchitis virus, strain Beaudette, have been cloned into pBR322 using cDNA primed with a specific oligonucleotide. A 5.3 kilobase viral insert in the clone pMB179 has been identified. The region of this clone coding for the spike gene has been sequenced by the chain termination method, and we present here the first report of DNA sequence data for a coronavirus spike protein, the protein which forms the characteristic 'corona' after which the group is named. The amino acid sequence of the primary translation product, deduced from the DNA sequence, predicts a polypeptide of 1162 amino acids with a molecular weight of 127 006. This has many interesting features which confirm and extend our knowledge of this recently characterized membrane glycoprotein. The polypeptide is subsequently cleaved to S1 and S2, and partial amino acid analysis of the amino-terminus of the S1 polypeptide has been employed to locate the position of this terminus of S1 within the large open reading frame. The amino acid analysis also reveals the presence of an 18 amino acid putative signal sequence on the primary translation product which is not present on the mature S1 polypeptide.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Base Sequence Cloning, Molecular Coronaviridae/ genetics Genes, Viral Infectious bronchitis virus/ genetics Viral Envelope Proteins/analysis/ genetics
Subjects: Investigative techniques and equipment > cloning
Investigative techniques and equipment > assays > cloning

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
organism description > virus
CSHL Authors:
Communities: CSHL labs > Pappin lab
Depositing User: Matt Covey
Date: April 1985
Date Deposited: 18 Sep 2014 19:25
Last Modified: 18 Sep 2014 19:25
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30746

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