Characterization of cDNA clones encoding the extrinsic 23 kDa polypeptide of the oxygen-evolving complex of photosystem II in pea

Wales, R., Newman, B. J., Rose, S. A., Pappin, D., Gray, J. C. (November 1989) Characterization of cDNA clones encoding the extrinsic 23 kDa polypeptide of the oxygen-evolving complex of photosystem II in pea. Plant Mol Biol, 13 (5). pp. 573-82. ISSN 0167-4412 (Print)0167-4412 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/2491674
DOI: 10.1007/BF00027317

Abstract

The 23 kDa polypeptide of the oxygen-evolving complex of photosystem II has been extracted from pea photosystem II particles by washing with 1 M NaCl and purified by anion-exchange chromatography. The N-terminal amino acid sequence has been determined and specific antisera have been raised in rabbits and used to screen a pea-leaf cDNA library in lambda gt11. Determination of the nucleotide sequence of two clones provided the nucleotide sequence for the full 23 kDa polypeptide. The deduced amino acid sequence showed it to code for a mature protein of 186 amino acid residues with an N-terminal presequence of 73 amino acid residues showing a high degree of conservation with previously reported 23 kDa sequences from spinach and Chlamydomonas. Southern blots of genomic DNA from pea probed with the labelled cDNA gave rise to only one band suggesting that the protein is encoded by a single gene. Northern blots of RNA extracted from various organs indicated a message of approximately 1.1 kb, in good agreement with the size of the cDNA, in all chlorophyll-containing tissues. Western blots of protein extracted from the same organs indicated that the 23 kDa polypeptide was present in all major organs of the plant except the roots.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Base Sequence Cloning, Molecular DNA/ genetics Fabaceae/genetics/metabolism Light-Harvesting Protein Complexes Molecular Sequence Data Photosynthetic Reaction Center Complex Proteins/ genetics/isolation & purification Photosystem II Protein Complex Plant Proteins Plants/ genetics/metabolism Plants, Medicinal RNA, Messenger/genetics Tissue Distribution
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > cDNA
organism description > plant
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > polypeptides
CSHL Authors:
Communities: CSHL labs > Pappin lab
Depositing User: Matt Covey
Date: 10 November 1989
Date Deposited: 18 Sep 2014 19:19
Last Modified: 18 Sep 2014 19:19
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30745

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