Deregulation of Cdc2 kinase induces caspase-3 activation and apoptosis

Gu, L., Zheng, H. W., Murray, S. A., Ying, H. Q., Xiao, Z. X. J. (March 2003) Deregulation of Cdc2 kinase induces caspase-3 activation and apoptosis. Biochemical and Biophysical Research Communications, 302 (2). pp. 384-391. ISSN 0006-291X

URL: http://www.ncbi.nlm.nih.gov/pubmed/12604359
DOI: 10.1016/s0006-291x(03)00189-x

Abstract

Progression of the cell cycle and control of apoptosis are tightly linked processes. It has been reported that manifestation of apoptosis requires cdc2 kinase activity yet the mechanism(s) of which is largely unclear. In an attempt to study the role of human MDM2 (HDM2) in interphase and mitosis, we employed the Xenopus cell-free system to study HDM2 protein stability. Interestingly, HDM2 is specifically cleaved in Xenopus mitotic extracts but not in the interphase extracts. We demonstrate that HDM2 cleavage is dependent on caspase-3 and that activation of cdc2 kinase results in caspase-3 activation in the Xenopus cell-free system. Furthermore, expression of cdc2 kinase in mammalian cells leads to activation of caspase-3 and apoptosis. Taken together, these data indicate that deregulation of cdc2 kinase activity can trigger apoptotic machinery that leads to caspase-3 activation and apoptosis. (C) 2003 Elsevier Science (USA). All rights reserved.

Item Type: Paper
Uncontrolled Keywords: cdc2 caspase apoptosis xenopus HDM2 FAS-INDUCED APOPTOSIS CYCLIN-DEPENDENT KINASES SOFT-TISSUE SARCOMAS CELL-CYCLE MDM2 ONCOPROTEIN UBIQUITIN LIGASE PROTEIN-KINASES UP-REGULATION P53 EXPRESSION Biochemistry & Molecular Biology Biophysics
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > apoptosis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > Protease > caspases
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > cdc2
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle
CSHL Authors:
Communities: CSHL labs > Zheng lab
Depositing User: Matt Covey
Date: March 2003
Date Deposited: 22 Aug 2014 15:51
Last Modified: 22 Aug 2014 15:51
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30711

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