Infection of frog neurons with vaccinia virus permits in vivo expression of foreign proteins

Wu, G. Y., Zou, D. J., Koothan, T., Cline, H. T. (1995) Infection of frog neurons with vaccinia virus permits in vivo expression of foreign proteins. Neuron, 14 (4). pp. 681-4. ISSN 0896-6273 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/7718230
DOI: 10.1016/0896-6273(95)90211-2

Abstract

Vaccinia virus can be used to infect cells in the CNS of frogs, Xenopus laevis, and Rana pipiens, both in vivo and in vitro. In vivo infections were accomplished by injection of viral solution into the tectal ventricle of stage 40-48 tadpoles or by local injections into distinct neural regions. Infections with high titer of virus injected into the ventricle resulted in the majority of cells in the brain expressing foreign protein, while cells in the retina and optic nerve showed no expression. Infection with lower viral titers resulted in fewer infected cells that were distributed throughout the otherwise normal tissue. Intense expression of foreign protein in the brain was observed 36 hr after injection and remained high for at least 4 days. Infected animals developed normally and had the same number of cells in the optic tectum as control animals. Infection with a recombinant virus carrying the gene for Green Fluorescent Protein labels neurons, so that infected cells can be observed in vivo. Vaccinia virus provides a versatile means to alter proteins in distinct populations of neurons in amphibia.

Item Type: Paper
Uncontrolled Keywords: Animals Brain/metabolism Gene Expression Green Fluorescent Proteins Humans Larva/metabolism Luminescent Proteins/genetics Neurons/ metabolism/virology Optic Nerve/metabolism Rana pipiens Research Support, Non-U.S. Gov't Retina/metabolism Superior Colliculus/metabolism Transfection Vaccinia virus/ genetics Xenopus laevis beta-Galactosidase/genetics
Subjects: organism description > animal > Frog
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > green fluorescent protein
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > recombination
organism description > virus
organism description > animal > Frog > xenopus
CSHL Authors:
Communities: CSHL labs > Cline lab
Depositing User: Jessica Koos
Date Deposited: 31 Jul 2014 16:07
Last Modified: 31 Jul 2014 16:07
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30653

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