The gene encoding the VP16-accessory protein HCF (HCFC1) resides in human Xq28 and is highly expressed in fetal tissues and the adult kidney

Wilson, A. C., Parrish, J. E., Massa, H. F., Nelson, D. L., Trask, B. J., Herr, W. (January 1995) The gene encoding the VP16-accessory protein HCF (HCFC1) resides in human Xq28 and is highly expressed in fetal tissues and the adult kidney. Genomics, 25 (2). pp. 462-8. ISSN 0888-7543 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/7789979
DOI: 10.1016/0888-7543(95)80046-O

Abstract

After herpes simplex virus (HSV) infection, the viral regulatory protein VP16 activates transcription of the HSV immediate-early promoters by directing complex formation with two cellular proteins, the POU-homeodomain transcription factor Oct-1 and the host cell factor HCF. The function of HCF in uninfected cells is unknown. Here we show by fluorescence in situ hybridization and somatic cell hybrid analysis that the gene encoding human HCF, HCFC1, maps to the q28 region of the X chromosome. Yeast artificial chromosome and cosmid mapping localizes the HCFC1 gene within 100 kb distal of the renal vasopressin type-2 receptor (V2R) gene and adjacent to the renin-binding protein gene (RENBP). The HCFC1 gene is apparently unique. HCF transcripts and protein are most abundant in fetal and placental tissues and cell lines, suggesting a role in cell proliferation. In adults, HCF protein is abundant in the kidney, but not in the brain, a site of latent HSV infection and where HCF levels may influence progression of HSV infection.

Item Type: Paper
Uncontrolled Keywords: Animals Base Sequence DNA, Complementary/genetics DNA-Binding Proteins/metabolism Fetal Proteins/biosynthesis/genetics Gene Expression Regulation Genes Herpes Simplex Virus Protein Vmw65/metabolism Host Cell Factor C1 Humans Hybrid Cells In Situ Hybridization, Fluorescence Kidney/ metabolism Molecular Sequence Data Octamer Transcription Factor-1 Organ Specificity Proteins/ genetics Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Transcription Factors/metabolism X Chromosome
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
organism description > animal > developmental stage > fetal
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
organism description > virus > herpes simplex virus
organism description > animal > mammal > primates > hominids > human
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor
CSHL Authors:
Communities: CSHL labs > Herr lab
Depositing User: Jessica Koos
Date: 20 January 1995
Date Deposited: 11 Aug 2014 20:40
Last Modified: 11 Aug 2014 20:40
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30650

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving