The phenylalanine ammonia-lyase gene family in Arabidopsis thaliana

Wanner, L. A., Li, G., Ware, D., Somssich, I. E., Davis, K. R. (January 1995) The phenylalanine ammonia-lyase gene family in Arabidopsis thaliana. Plant Mol Biol, 27 (2). pp. 327-38. ISSN 0167-4412 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/7888622
DOI: 10.1007/BF00020187

Abstract

Phenylpropanoid derivatives are a complex class of secondary metabolites that have many important roles in plants during normal growth and in responses to environmental stress. Phenylalanine ammonialyase (PAL) catalyzes the first step in the biosynthesis of phenylpropanoids, and is usually encoded by a multi-gene family. Genomic clones for three Arabidopsis thaliana PAL genes containing the entire protein-coding region and upstream and downstream sequences have been obtained and completely sequenced. Two A. thaliana PAL genes (PAL1 and PAL2) are structurally similar to PAL genes that have been cloned from other plant species, with a single intron at a conserved position, and a long highly conserved second exon. Previously identified promoter motifs plus several additional sequence motifs were found in the promoter regions of PAL1 and PAL2. Expression of PAL1 and PAL2 is both qualitatively and quantitatively similar in different plant organs and under various inductive conditions. A third A. thaliana PAL gene, PAL3, differs significantly from PAL1 and PAL2 and other sequenced plant PAL genes. PAL3 contains an additional intron, and its deduced amino acid sequence is less homologous to other PAL proteins. The PAL3 promoter region lacks several sequence motifs conserved between A. thaliana PAL1 and PAL2, as well as motifs described in other genes involved in phenylpropanoid metabolism. A. thaliana PAL3 was expressed at very low levels under the conditions examined.

Item Type: Paper
Uncontrolled Keywords: Arabidopsis/enzymology/ genetics Base Sequence Cloning, Molecular Exons/genetics Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Genes, Plant/genetics Introns/genetics Molecular Sequence Data Multigene Family/ genetics Phenylalanine Ammonia-Lyase/ genetics Phylogeny Promoter Regions, Genetic/genetics RNA Splicing/genetics RNA, Messenger/analysis RNA, Plant/analysis Restriction Mapping Sequence Analysis, DNA Sequence Homology, Amino Acid Transcription, Genetic/genetics
Subjects: organism description > plant > Arabidopsis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA splicing
CSHL Authors:
Communities: CSHL labs > Ware lab
Depositing User: Jessica Koos
Date: January 1995
Date Deposited: 11 Aug 2014 20:45
Last Modified: 11 Aug 2014 20:45
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30648

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