Patterns of Gene-Action in Plant Development Revealed by Enhancer Trap and Gene Trap Transposable Elements

Sundaresan, V., Springer, P., Volpe, T., Haward, S., Jones, J. D. G., Dean, C., Ma, H., Martienssen, R. (July 1995) Patterns of Gene-Action in Plant Development Revealed by Enhancer Trap and Gene Trap Transposable Elements. Genes & development, 9 (14). pp. 1797-1810. ISSN 0890-9369

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URL: http://www.ncbi.nlm.nih.gov/pubmed/7622040
DOI: 10.1101/gad.9.14.1797

Abstract

The crucifer Arabidopsis thaliana has been used widely as a model organism for the study of plant development. We describe here the development of an efficient insertional mutagenesis system in Arabidopsis that permits identification of genes by their patterns of expression during development. Transposable elements of the Ac/Ds system carrying the GUS reporter gene have been designed to act as enhancer traps or gene traps. A novel selection scheme maximizes recovery of unlinked transposition events. In this study 491 plants carrying independent transposon insertions were generated and screened for expression patterns. One-half of the enhancer trap insertions and one-quarter of the gene trap insertions displayed GUS expression in seedlings or flowers, including expression patterns specific to organs, tissues, cell types, or developmental stages. The patterns identify genes that act during organogenesis, pattern formation, or cell differentiation. Transposon insertion lines with specific GUS expression patterns provide valuable markers for studies of Arabidopsis development and identify new cell types or subtypes in plants. The diversity of gene expression patterns generated suggests that the identification and cloning of Arabidopsis genes expressed in any developmental process is feasible using this system.

Item Type: Paper
Uncontrolled Keywords: arabidopsis development plant development enhancer trap gene trap transposable elements embryonic stem-cells arabidopsis-thaliana molecular-cloning agrobacterium-tumefaciens transposition pattern regulated genes expression drosophila activator excision
Subjects: organism description > plant > Arabidopsis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function
organism description > plant
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA expression > transposable elements
CSHL Authors:
Communities: CSHL labs > Martienssen lab
Depositing User: Jessica Koos
Date: 15 July 1995
Date Deposited: 12 Aug 2014 16:10
Last Modified: 12 Aug 2014 16:10
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30643

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