Mutations associated with familial melanoma impair p16(INK4) function

Ranade, K., Hussussian, C. J., Sikorski, R. S., Varmus, H. E., Goldstein, A. M., Tucker, M. A., Serrano, M., Hannon, G. J., Beach, D., Dracopoli, N. C. (1995) Mutations associated with familial melanoma impair p16(INK4) function. Nature Genetics, 10 (1). pp. 114-116. ISSN 10614036 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/7647780
DOI: 10.1038/ng0595-114

Abstract

Cell division is controlled by a series of positive and negative regulators which act at sequential points throughout the cell cycle. Disturbance of these checks could contribute to cancer by allowing excessive cell proliferation. The point in G1 at which cells irrevocably commit to DNA synthesis is controlled by protein complexes consisting of cyclin-dependent kinases (CDK4 or CDK6) and cyclins (D1, D2 or D3). These complexes are inhibited by low molecular weight proteins, such as pl6(INK4) (refs 1,2), p15(INK4B) (ref. 3) and pl 8 (ref. 4). Deletion or mutation of these CDK- inhibitors could lead to unchecked cell growth, suggesting that members of the p16(INK4) family may be tumour suppressor genes. The recent detection of p16(INK4) (MTS1) mutations in familial melanoma kindreds, many human tumour cell lines, and primary tumours is consistent with this idea. Previously, we described eight germline p16(INK4) substitutions in 18 familial melanoma kindreds. Genetic analyses suggested that five mutations predisposed carriers to melanoma, whereas two missense mutations had no phenotypic effect. We now describe biochemical analyses of the missense germline mutations and a single somatic mutation detected in these families. Only the melanoma-predisposing mutants were impaired in their ability to inhibit the catalytic activity of the cyclin D1/CDK4 and cyclin D1/CDK6 complexes in vitro. Our data provide a biochemical rationale for the hypothesis that carriers of certain p16(INK4) mutations are at increased risk of developing melanoma.

Item Type: Paper
Uncontrolled Keywords: article cancer risk cell division cell proliferation enzyme activity familial disease gene mutation genetic linkage genetic polymorphism human melanoma priority journal tumor suppressor gene Animal Carrier Proteins Cell Line Cyclin D1 Cyclins Insects Mutagenesis, Site-Directed Mutation Oncogene Proteins Phosphorylation Protein Binding Protein p16 Protein-Serine-Threonine Kinases Recombinant Fusion Proteins Support, Non-U.S. Gov't
Subjects: organism description > animal > mammal > primates > hominids > human
diseases & disorders > cancer > cancer types > melanomas
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations
CSHL Authors:
Communities: CSHL labs > Beach lab
CSHL labs > Hannon lab
Depositing User: Jessica Koos
Date: 1995
Date Deposited: 08 Aug 2014 20:57
Last Modified: 08 Aug 2014 20:57
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30636

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