Pettit, D. L., Koothan, T., Liao, D., Malinow, R. (April 1995) Vaccinia virus transfection of hippocampal slice neurons. Neuron, 14 (4). pp. 685-8.
Abstract
Here we describe a technique that uses a recombinant vaccinia virus to transfect neurons in rat hippocampal slices. This technique allows the use of molecular biological manipulations on neuronal tissue while maintaining intact synaptic function. This method should be useful in testing specific hypotheses regarding the role of synaptic proteins.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Animals Dendrites/metabolism Electrophysiology Fluoresceins/metabolism Fluorescent Dyes Galactosides/metabolism Glutathione/metabolism Hippocampus/*cytology Neurons/*metabolism/physiology Rats Rats, Sprague-Dawley Recombinant Proteins/analysis *Transfection Vaccinia virus/*genetics beta-Galactosidase/genetics |
| Subjects: | organs, tissues, organelles, cell types and functions > tissues types and functions > hippocampus organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organism description > animal > mammal > rodent > rat organism description > animal > mammal > rodent > rat organism description > virus |
| CSHL Authors: | |
| Communities: | CSHL labs > Malinow lab |
| Depositing User: | Jessica Koos |
| Date: | April 1995 |
| Date Deposited: | 11 Aug 2014 18:43 |
| Last Modified: | 11 Aug 2014 18:43 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/30632 |
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