Vaccinia virus transfection of hippocampal slice neurons

Pettit, D. L., Koothan, T., Liao, D., Malinow, R. (1995) Vaccinia virus transfection of hippocampal slice neurons. Neuron, 14 (4). pp. 685-8.

URL: http://www.ncbi.nlm.nih.gov/pubmed/7718231
DOI: 10.1016/0896-6273(95)90212-0

Abstract

Here we describe a technique that uses a recombinant vaccinia virus to transfect neurons in rat hippocampal slices. This technique allows the use of molecular biological manipulations on neuronal tissue while maintaining intact synaptic function. This method should be useful in testing specific hypotheses regarding the role of synaptic proteins.

Item Type: Paper
Uncontrolled Keywords: Animals Dendrites/metabolism Electrophysiology Fluoresceins/metabolism Fluorescent Dyes Galactosides/metabolism Glutathione/metabolism Hippocampus/*cytology Neurons/*metabolism/physiology Rats Rats, Sprague-Dawley Recombinant Proteins/analysis *Transfection Vaccinia virus/*genetics beta-Galactosidase/genetics
Subjects: organs, tissues, organelles, cell types and functions > tissues types and functions > hippocampus
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons

organism description > animal > mammal > rodent > rat
organism description > animal > mammal > rodent > rat

organism description > virus
CSHL Authors:
Communities: CSHL labs > Malinow lab
Depositing User: Jessica Koos
Date Deposited: 11 Aug 2014 18:43
Last Modified: 11 Aug 2014 18:43
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30632

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving