O'Gara, M., McCloy, K., Malone, T., Cheng, X. (May 1995) Structure-based sequence alignment of three AdoMet-dependent DNA methyltransferases. Gene, 157 (1-2). pp. 135-8. ISSN 0378-1119 (Print)
Abstract
M.HhaI, M.TaqI and COMT are DNA methyltransferases (MTases) which catalyze the transfer of a methyl group from the cofactor AdoMet to C5 of cytosine, to N6 of adenine and to a hydroxyl group of catechol, respectively. The larger catalytic domains of the bilobal proteins, M.HhaI and M.TaqI, and the entire single domain of COMT have an alpha/beta structure containing a mixed central beta-sheet. These domains have very similar folding. By allowing appropriate 'insertions' or 'deletions' in the backbones of the three structures, it was possible to find more conserved motifs in M.TaqI and COMT. The similarity in protein folding and the equivalence of amino-acid sequences revealed by the structural alignment indicate that many AdoMet-dependent MTases may share a common catalytic domain structure.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Amino Acid Sequence Binding Sites Catechol O-Methyltransferase/ chemistry/metabolism Comparative Study Crystallography, X-Ray Molecular Sequence Data Protein Conformation Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. S-Adenosylmethionine/ metabolism Sequence Homology, Amino Acid Site-Specific DNA Methyltransferase (Cytosine-Specific)/ chemistry/metabolism Site-Specific DNA-Methyltransferase (Adenine-Specific)/ chemistry/metabolism Structure-Activity Relationship |
| Subjects: | bioinformatics > genomics and proteomics > design > amino acid design bioinformatics > genomics and proteomics > alignment > sequence alignment bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > methyltransferase bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types Investigative techniques and equipment > x ray crystallography |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Jessica Koos |
| Date: | 19 May 1995 |
| Date Deposited: | 11 Aug 2014 20:32 |
| Last Modified: | 11 Aug 2014 20:32 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/30627 |
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