Effects of the simian virus 40 origin of replication on transcription from the human immunodeficiency virus type 1 promoter

Nahreini, P., Mathews, M. B. (February 1995) Effects of the simian virus 40 origin of replication on transcription from the human immunodeficiency virus type 1 promoter. J Virol, 69 (2). pp. 1296-301. ISSN 0022-538X (Print)

[img]
Preview
PDF
J. Virol.-1995-Nahreini-1296-301.pdf - Published Version

Download (319Kb) | Preview
URL: http://www.ncbi.nlm.nih.gov/pubmed/7815509

Abstract

Positive and negative effects of DNA replication on gene transcription have been documented in a variety of systems. We examined the effects of the simian virus 40 (SV40) origin of replication on transcription from the human immunodeficiency virus type 1 (HIV-1) promoter, using a transient expression assay in COS-1 cells. The basal activity and Tat transactivation of the HIV promoter were greatly stimulated by the SV40 origin of replication independent of its position relative to the long terminal repeat. These effects were abolished by mutational inactivation of the SV40 origin and were reduced by a DNA replication inhibitor. The magnitude of promoter activation exceeded the increment expected from the increase in template number resulting from DNA replication. The SV40 T-antigen-induced DNA replication augmented the generation of both processive and nonprocessive HIV long terminal repeat-directed transcripts, and Tat primarily enhanced the initiation of those transcripts that were destined to be efficiently elongated. Our data suggest that the HIV promoter displays greater transcriptional activity on replicative DNA templates. This property may influence the activity of integrated HIV provirus and its transition from latency to productive infection.

Item Type: Paper
Uncontrolled Keywords: Cell Line DNA Replication Gene Expression HIV Long Terminal Repeat HIV-1/ genetics Mutation Promoter Regions (Genetics) Research Support, U.S. Gov't, P.H.S. Simian virus 40/ genetics Transcription, Genetic Virus Replication
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
diseases & disorders > viral diseases > HIV
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
organism description > animal > mammal > primates > monkey
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations
organism description > virus
CSHL Authors:
Communities: CSHL labs
Depositing User: Jessica Koos
Date: February 1995
Date Deposited: 08 Aug 2014 19:40
Last Modified: 08 Aug 2014 19:40
PMCID: PMC188708
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30624

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving