Disruption of the target G-C base-pair by the HhaI methyltransferase

Klimasauskas, S., Roberts, R. J. (May 1995) Disruption of the target G-C base-pair by the HhaI methyltransferase. Gene, 157 (1-2). pp. 163-4. ISSN 0378-1119 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/7607483
DOI: 10.1016/0378-1119(94)00624-2

Abstract

HhaI MTase binds DNA duplexes containing mismatches at the target base position with higher affinity than that observed for the canonical substrate. The stability of these MTase-DNA complexes inversely correlates with the strength of the base pair that is disrupted upon interaction. This finding may offer a general tool to detect other enzymes that flip bases out of the DNA helix.

Item Type: Paper
Uncontrolled Keywords: Base Composition Base Sequence Comparative Study Cytosine DNA/chemistry/ metabolism Guanine Molecular Sequence Data Site-Specific DNA Methyltransferase (Cytosine-Specific)/ metabolism Substrate Specificity
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > methyltransferase
CSHL Authors:
Communities: CSHL labs > Roberts lab
Depositing User: Jessica Koos
Date: 19 May 1995
Date Deposited: 12 Aug 2014 14:46
Last Modified: 12 Aug 2014 14:46
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30610

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