Overexpression of SIS2, which contains an extremely acidic region, increases the expression of SWI4, CLN1 and CLN2 in sit4 mutants

Di Como, C. J., Bose, R., Arndt, K. T. (1995) Overexpression of SIS2, which contains an extremely acidic region, increases the expression of SWI4, CLN1 and CLN2 in sit4 mutants. Genetics, 139 (1). pp. 95-107. ISSN 0016-6731 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/7705654

Abstract

The Saccharomyces cerevisiae SIS2 gene was identified by its ability, when present on a high copy number plasmid, to increase dramatically the growth rate of sit4 mutants. SIT4 encodes a type 2A-related protein phosphatase that is required in late G1 for normal G1 cyclin expression and for bud initiation. Overexpression of SIS2, which contains an extremely acidic carboxyl terminal region, stimulated the rate of CLN1, CLN2, SWI4 and CLB5 expression in sit4 mutants. Also, overexpression of SIS2 in a CLN1 cln2 cln3 strain stimulated the growth rate and the rate of CLN1 and CLB5 RNA accumulation during late G1. The SIS2 protein fractionated with nuclei and was released from the nuclear fraction by treatment with either DNase I or micrococcal nuclease, but not by RNase A. This result, combined with the finding that overexpression of SIS2 is extremely to a strain containing lower than normal levels of histones H2A and H2B, suggests that SIS2 might function to stimulate transcription via an interaction with chromatin.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Base Sequence Cell Compartmentation Cell Cycle/ genetics Cell Cycle Proteins Cell Nucleus/chemistry Chromatin/physiology Cyclins/ biosynthesis Fungal Proteins/ biosynthesis/ genetics Gene Deletion Gene Expression Regulation, Fungal Genes, Fungal/genetics Molecular Sequence Data Mutation Phosphoprotein Phosphatase/genetics Research Support, U.S. Gov't, P.H.S. Saccharomyces cerevisiae/ genetics/growth & development Saccharomyces cerevisiae Proteins Sequence Homology, Amino Acid Transcription Factors Transcription, Genetic
Subjects: bioinformatics > genomics and proteomics > design > amino acid design
organism description > yeast > Saccharomyces
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor
CSHL Authors:
Communities: CSHL labs
Depositing User: Jessica Koos
Date Deposited: 15 Aug 2014 19:56
Last Modified: 15 Aug 2014 19:56
PMCID: PMC1206351
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30589

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving