Di Como, C. J., Bose, R., Arndt, K. T.
(January 1995)
Overexpression of SIS2, which contains an extremely acidic region, increases the expression of SWI4, CLN1 and CLN2 in sit4 mutants.
Genetics, 139 (1).
pp. 95-107.
ISSN 0016-6731 (Print)
Abstract
The Saccharomyces cerevisiae SIS2 gene was identified by its ability, when present on a high copy number plasmid, to increase dramatically the growth rate of sit4 mutants. SIT4 encodes a type 2A-related protein phosphatase that is required in late G1 for normal G1 cyclin expression and for bud initiation. Overexpression of SIS2, which contains an extremely acidic carboxyl terminal region, stimulated the rate of CLN1, CLN2, SWI4 and CLB5 expression in sit4 mutants. Also, overexpression of SIS2 in a CLN1 cln2 cln3 strain stimulated the growth rate and the rate of CLN1 and CLB5 RNA accumulation during late G1. The SIS2 protein fractionated with nuclei and was released from the nuclear fraction by treatment with either DNase I or micrococcal nuclease, but not by RNase A. This result, combined with the finding that overexpression of SIS2 is extremely to a strain containing lower than normal levels of histones H2A and H2B, suggests that SIS2 might function to stimulate transcription via an interaction with chromatin.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
Amino Acid Sequence
Base Sequence
Cell Compartmentation
Cell Cycle/ genetics
Cell Cycle Proteins
Cell Nucleus/chemistry
Chromatin/physiology
Cyclins/ biosynthesis
Fungal Proteins/ biosynthesis/ genetics
Gene Deletion
Gene Expression Regulation, Fungal
Genes, Fungal/genetics
Molecular Sequence Data
Mutation
Phosphoprotein Phosphatase/genetics
Research Support, U.S. Gov't, P.H.S.
Saccharomyces cerevisiae/ genetics/growth & development
Saccharomyces cerevisiae Proteins
Sequence Homology, Amino Acid
Transcription Factors
Transcription, Genetic |
| Subjects: |
bioinformatics > genomics and proteomics > design > amino acid design
organism description > yeast > Saccharomyces
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor |
| CSHL Authors: |
|
| Communities: |
CSHL labs |
| Depositing User: |
Jessica Koos
|
| Date: |
January 1995 |
| Date Deposited: |
15 Aug 2014 19:56 |
| Last Modified: |
15 Aug 2014 19:56 |
| PMCID: |
PMC1206351 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/30589 |
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