RACH2, a novel human gene that complements a fission yeast cell cycle checkpoint mutation

Davey, S., Beach, D. (1995) RACH2, a novel human gene that complements a fission yeast cell cycle checkpoint mutation. Mol Biol Cell, 6 (10). pp. 1411-21. ISSN 1059-1524 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/8573795
DOI: 10.1091/mbc.6.10.1411

Abstract

We have identified a novel human gene by virtue of its ability to complement the rad1-1 checkpoint mutant of Schizosaccharomyces pombe. This gene, called RACH2, rescues the temperature-sensitive lethality of a rad1-1 wee1-50 double mutant of S. pombe. Expression of RACH2 in S. pombe rad1-1 strains partially restores UV resistance to the rad1-1 mutant strain. Expression of RACH2 in a rad1-1 cdc25-22 double mutant partially restores the dose-dependent delay in mitotic entry after irradiation that is lost in rad1-1 checkpoint-deficient mutants. Overexpression of RACH2 in human tissue culture cells induces apoptosis.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Apoptosis Cell Cycle Cell Cycle Proteins/genetics Cell Line Cloning, Molecular DNA, Antisense DNA-Binding Proteins Endonucleases/ genetics Fibroblasts Fungal Proteins/biosynthesis/chemistry/ genetics/ physiology Genes/ genetics Genetic Complementation Test Humans Keratinocytes Membrane Transport Proteins Molecular Sequence Data Mutation Phosphoprotein Phosphatase/genetics Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Schizosaccharomyces/ cytology/genetics/radiation effects Schizosaccharomyces pombe Proteins Sequence Analysis, DNA Sequence Homology, Amino Acid Ultraviolet Rays cdc25 Phosphatase
Subjects: bioinformatics > genomics and proteomics > design > amino acid design
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > apoptosis
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle
Investigative techniques and equipment > cloning
Investigative techniques and equipment > assays > cloning

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > fibroblasts
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > fibroblasts
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > fibroblasts

organism description > animal > mammal > primates > hominids > human
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations
CSHL Authors:
Communities: CSHL labs > Beach lab
Depositing User: Jessica Koos
Date Deposited: 12 Aug 2014 13:58
Last Modified: 12 Aug 2014 13:58
PMCID: PMC301296
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30587

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