Basal promoter elements as a selective determinant of transcriptional activator function

Das, G., Hinkley, C. S., Herr, W. (April 1995) Basal promoter elements as a selective determinant of transcriptional activator function. Nature, 374 (6523). pp. 657-60. ISSN 0028-0836 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/7715708
DOI: 10.1038/374657a0

Abstract

In eukaryotes, activation of transcription involves an interplay between activators bound to cis-regulatory elements and factors bound to basal elements near the start site of transcription. The basal elements, for example the TATA box or proximal sequence element (PSE) of small nuclear RNA (snRNA) promoters, nucleate the assembly of basal transcription complexes, components of which interact with activators. Although one basal transcription complex can interact with many activators, it is unclear whether different basal transcription complexes can direct different responses to particular activators. We show here that changing the arrangement of basal elements can alter the response to transcriptional activation domains. Indeed, in the human U6 snRNA promoter, point mutation of either a TATA box or PSE results in diametrically opposed responses to VP16- and Sp1-derived activation domains. These basal elements can even discriminate small changes in an activation domain. Thus the arrangement of basal promoter elements provides a mechanism for differential regulation of transcription.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence DNA/metabolism Fungal Proteins/metabolism Hela Cells Humans Molecular Sequence Data Promoter Regions (Genetics) Proto-Oncogene Proteins c-fos/genetics RNA, Small Nuclear/genetics Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Saccharomyces cerevisiae Proteins TATA Box Trans-Activation (Genetics) Transcription Factors/metabolism
Subjects: bioinformatics > genomics and proteomics > design > amino acid design
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
organism description > yeast > Saccharomyces
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
organism description > animal > mammal > primates > hominids > human
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor
CSHL Authors:
Communities: CSHL labs > Herr lab
Depositing User: Jessica Koos
Date: 13 April 1995
Date Deposited: 12 Aug 2014 14:12
Last Modified: 12 Aug 2014 14:12
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30586

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