The Saccharomyces cerevisiae HIS3 and LYS2 genes complement the Schizosaccharomyces pombe his5-303 and lys1-131 mutations, respectively: new selectable markers and new multi-purpose multicopy shuttle vectors, pSP3 and pSP4

Cottarel, G. (September 1995) The Saccharomyces cerevisiae HIS3 and LYS2 genes complement the Schizosaccharomyces pombe his5-303 and lys1-131 mutations, respectively: new selectable markers and new multi-purpose multicopy shuttle vectors, pSP3 and pSP4. Curr Genet, 28 (4). pp. 380-3. ISSN 0172-8083 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/8590485

Abstract

Three new S. pombe plasmids are described. Plasmids pSP3 and pSP4 are two Schizosaccharomyces pombe ars1 multicopy vectors with the Saccharomyces cerevisiae HIS3 or LYS2 genes as selectable markers. They complement the S. pombe his5-303 or lys1-131 mutations, respectively. Plasmid pSPars1 is a vector carrying the S. pombe ars1 and a unique NdeI site which allows the introduction of any selectable marker therefore bringing a unified vector backbone for the construction of new S. pombe/S. cerevisiae/E. coli shuttle vectors. These plasmids permit classical molecular genetic techniques to be performed directly.

Item Type: Paper
Uncontrolled Keywords: Aldehyde Oxidoreductases/genetics Fungal Proteins/genetics Gene Dosage Genes, Fungal/ genetics Genetic Complementation Test Genetic Markers/genetics Genetic Vectors/ genetics Genotype Hydro-Lyases/genetics L-Aminoadipate-Semialdehyde Dehydrogenase Mutation/genetics Plasmids/genetics Research Support, Non-U.S. Gov't Saccharomyces cerevisiae/ genetics Schizosaccharomyces/ genetics
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing
organism description > yeast > Saccharomyces
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > plasmid
organism description > yeast > Saccharomyces > Schizosaccharomyces pombe
CSHL Authors:
Communities: CSHL labs > Beach lab
Depositing User: Jessica Koos
Date: September 1995
Date Deposited: 12 Aug 2014 14:36
Last Modified: 12 Aug 2014 14:36
URI: http://repository.cshl.edu/id/eprint/30584

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