Purification of Tetrahymena telomerase and cloning of genes encoding the two protein components of the enzyme

Collins, K., Kobayashi, R., Greider, C. W. (June 1995) Purification of Tetrahymena telomerase and cloning of genes encoding the two protein components of the enzyme. Cell, 81 (5). pp. 677-86. ISSN 0092-8674 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/7774009
DOI: 10.1016/0092-8674(95)90529-4

Abstract

Telomerase is a ribonucleoprotein DNA polymerase that catalyzes the de novo synthesis of telomeric simple sequence repeats. We describe the purification of telomerase and the cloning of cDNAs encoding two protein subunits from the ciliate Tetrahymena. Two proteins of 80 and 95 kDa copurified and coimmunoprecipitated with telomerase activity and the previously identified Tetrahymena telomerase RNA. The p95 subunit specifically cross-linked to a radiolabeled telomeric DNA primer, while the p80 subunit specifically bound to radiolabeled telomerase RNA. At the primary sequence level, the two telomerase proteins share only limited homologies with other polymerases and polymerase accessory factors.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Animals Antibodies, Protozoan Base Sequence Cloning, Molecular DNA Nucleotidylexotransferase/ genetics/immunology DNA, Complementary/genetics Electrophoresis, Polyacrylamide Gel Genes, Protozoan/ genetics Immunoblotting Molecular Sequence Data Nucleic Acid Conformation Precipitin Tests Protein Conformation Protozoan Proteins/ genetics RNA, Protozoan/metabolism Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Ribonucleoproteins/ genetics/isolation & purification Sequence Analysis Tetrahymena thermophila/enzymology/ genetics/isolation & purification
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > design > nucleic acid design
organism description > animal
Investigative techniques and equipment > cloning
Investigative techniques and equipment > assays > cloning

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > telomerase
CSHL Authors:
Communities: CSHL labs
Depositing User: Jessica Koos
Date: 2 June 1995
Date Deposited: 14 Aug 2014 16:03
Last Modified: 14 Aug 2014 16:03
URI: http://repository.cshl.edu/id/eprint/30581

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