Molecular cloning of linotte in Drosophila: a novel gene that functions in adults during associative learning

Bolwig, G. M., Del Vecchio, M., Hannon, G., Tully, T. (October 1995) Molecular cloning of linotte in Drosophila: a novel gene that functions in adults during associative learning. Neuron, 15 (4). pp. 829-42. ISSN 0896-6273 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/7576632
DOI: 10.1016/0896-6273(95)90174-4

Abstract

The linotte (lio) gene was identified in a screen for mutations that disrupted 3 hr memory after olfactory associative learning, without affecting the perception of odors or electroshock. The mutagenesis yielded a transposon-tagged gene disruption, which allowed rapid cloning of genomic DNA. The lio transcription unit was identified via rescue of the lio1 learning/memory defect by induced expression of a lio+ transgene in adults. The perception of odors or electroshock remained normal when the lio+ transgene was expressed in these lio1 flies. Learning/memory remained normal when the lio+ transgene was expressed in wild-type (lio+) flies. The lio gene produces only one transcript, the level of expression of which varies throughout development. Sequence analysis indicates that lio encodes a novel protein.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Animals Animals, Genetically Modified Blotting, Southern Chromosome Mapping Cloning, Molecular DNA Probes DNA Transposable Elements Drosophila/embryology/ genetics/physiology Drosophila Proteins Genes, Insect Heat Larva/metabolism Learning/ physiology Memory/physiology Molecular Sequence Data Proteins/chemistry/ genetics/physiology Pupa/metabolism Receptor Protein-Tyrosine Kinases Research Support, Non-U.S. Gov't Restriction Mapping Smell
Subjects: organism description > animal > insect > Drosophila
bioinformatics > genomics and proteomics > design > protein design
organism description > animal > developmental stage > adult
organism description > animal
Investigative techniques and equipment > cloning
Investigative techniques and equipment > assays > cloning

Investigative techniques and equipment > probes > DNA probes
organism description > animal behavior > learning
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > linotte gene
organism description > animal behavior > memory
CSHL Authors:
Communities: CSHL labs > Tully lab
Depositing User: Jessica Koos
Date: October 1995
Date Deposited: 15 Aug 2014 16:28
Last Modified: 15 Aug 2014 16:28
URI: http://repository.cshl.edu/id/eprint/30570

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