Functional characterization and developmental regulation of mouse telomerase RNA

Blasco, M. A., Funk, W., Villeponteau, B., Greider, C. W. (1995) Functional characterization and developmental regulation of mouse telomerase RNA. Science, 269 (5228). pp. 1267-70. ISSN 0036-8075 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/7544492
DOI: 10.1126/science.7544492

Abstract

Telomerase synthesizes telomeric DNA repeats onto chromosome ends de novo. The mouse telomerase RNA component was cloned and contained only 65 percent sequence identity with the human telomerase RNA. Alteration of the template region in vivo generated altered telomerase products. The shorter template regions of the mouse and other rodent telomerase RNAs could account for the shorter distribution of products (processivity) generated by the mouse enzyme relative to the human telomerase. Amounts of telomerase RNA increased in immortal cells derived from primary mouse fibroblasts. RNA was detected in all newborn mouse tissues tested but was decreased during postnatal development.

Item Type: Paper
Uncontrolled Keywords: 3T3 Cells Animals Animals, Newborn Base Sequence Brain/enzymology Cell Line, Transformed Cloning, Molecular DNA Nucleotidylexotransferase/antagonists & inhibitors/chemistry/genetics/ metabolism Gene Expression Regulation, Developmental Humans Kidney/enzymology Liver/enzymology Mice Molecular Sequence Data Muridae Mutagenesis Oligonucleotides, Antisense/pharmacology RNA/chemistry/genetics/ metabolism Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Templates, Genetic Transfection
Subjects: bioinformatics > genomics and proteomics > Mapping and Rendering > RNA Structure Rendering
organism description > animal
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > cell line
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > cell line
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > cell line

Investigative techniques and equipment > cloning
Investigative techniques and equipment > assays > cloning

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
organism description > animal > mammal > primates > hominids > human
organs, tissues, organelles, cell types and functions > organs types and functions > liver
organism description > animal > mammal > rodent > mouse
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations > mutagenesis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > telomerase
Investigative techniques and equipment > transfection
CSHL Authors:
Communities: CSHL labs
Depositing User: Jessica Koos
Date Deposited: 15 Aug 2014 16:40
Last Modified: 15 Aug 2014 16:40
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30568

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