Boundary elements of the Tetrahymena telomerase RNA template and alignment domains

Autexier, C., Greider, C. W. (September 1995) Boundary elements of the Tetrahymena telomerase RNA template and alignment domains. Genes Dev, 9 (18). pp. 2227-39. ISSN 0890-9369 (Print)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/7557377
DOI: 10.1101/gad.9.18.2227

Abstract

Telomerase is a DNA polymerase fundamental to the replication and maintenance of telomere sequences at chromosome ends. The RNA component of telomerase is essential for the synthesis of telomere repeats. In vitro, the template domain (5'-CAACCCCAA-3') of the Tetrahymena telomerase RNA dictates the addition of Tetrahymena-specific telomere repeats d(TTGGGG)n, onto the 3' end of G-rich or telomeric substrates that are base-paired with the template and alignment regions of the RNA. Using a reconstituted in vitro system, we determined that altering the sequence of the alignment and template domains affects processivity of telomerase without abolishing telomerase activity. These results suggest that alternative template/alignment regions may be functional. In the ciliate telomerase RNAs, there is a conserved sequence 5'-(CU)GUCA-3', located two residues upstream of the template domain. The location and sequence of this conserved domain defined the 5' boundary of the template region. These data provide insights into the regulation of telomere synthesis by telomerase.

Item Type: Paper
Uncontrolled Keywords: Animals Base Sequence Conserved Sequence DNA Replication DNA, Protozoan/biosynthesis Molecular Sequence Data Mutagenesis, Site-Directed Nucleic Acid Conformation RNA, Protozoan/ genetics Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Ribonucleoproteins/metabolism Sequence Alignment Structure-Activity Relationship Telomerase/ genetics/ metabolism Tetrahymena/enzymology/ genetics
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
bioinformatics > genomics and proteomics > design > nucleic acid design
organism description > animal
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations > mutagenesis
CSHL Authors:
Communities: CSHL labs > Greider lab
Depositing User: Jessica Koos
Date: 15 September 1995
Date Deposited: 15 Aug 2014 18:55
Last Modified: 15 Aug 2014 18:55
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30564

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