Two waves of de novo methylation during mouse germ cell development

Molaro, A., Falciatori, I., Hodges, E., Aravin, A. A., Marran, K., Rafii, S., McCombie, W. R., Smith, A. D., Hannon, G. J. (2014) Two waves of de novo methylation during mouse germ cell development. Genes and Development, 28 (14). pp. 1544-9. ISSN 08909369 (ISSN)

[img]
Preview
PDF (Paper)
Hannon and McCombie Genes & Development 2014.pdf - Published Version

Download (1204Kb) | Preview
URL: http://www.ncbi.nlm.nih.gov/pubmed/25030694
DOI: 10.1101/gad.244350.114

Abstract

During development, mammalian germ cells reprogram their epigenomes via a genome-wide erasure and de novo rewriting of DNA methylation marks. We know little of how methylation patterns are specifically determined. The piRNA pathway is thought to target the bulk of retrotransposon methylation. Here we show that most retrotransposon sequences are modified by default de novo methylation. However, potentially active retrotransposon copies evade this initial wave, likely mimicking features of protein-coding genes. These elements remain transcriptionally active and become targets of piRNA-mediated methylation. Thus, we posit that these two waves play essential roles in resetting germ cell epigenomes at each generation.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA methylation
organism description > animal > developmental stage
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > germ cell
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > germ cell
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > germ cell

organism description > animal > mammal > rodent > mouse
CSHL Authors:
Communities: CSHL Cancer Center Program > Cancer Genetics
CSHL labs > Hannon lab
CSHL labs > McCombie lab
CSHL Cancer Center Shared Resources > DNA Sequencing Service
Depositing User: Matt Covey
Date Deposited: 25 Jul 2014 14:49
Last Modified: 04 Nov 2015 20:30
PMCID: PMC4102761
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30554

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving