Rotation-mediated aggregation of skin fibroblasts in Duchenne muscular dystrophy. Effects of monensin

Witkowski, J. A., Statham, H. E., Dubowitz, V. (October 1983) Rotation-mediated aggregation of skin fibroblasts in Duchenne muscular dystrophy. Effects of monensin. Journal of the neurological sciences, 61 (3). pp. 425-33. ISSN 0022-510X (Print)0022-510x

URL: http://www.ncbi.nlm.nih.gov/pubmed/6655491

Abstract

Rotation-mediated aggregation of human skin fibroblasts has been studied and the patterns of aggregation compared between cultures obtained from 9 patients with Duchenne muscular dystrophy and from 10 normal controls. The rate of aggregation was dependent on the growth state of the cells, with growing cells aggregating more rapidly than growth-arrested cells. There was considerable variation between individuals in the rate at which cells aggregated but no differences were detected in the aggregation of normal and DMD cells measured by this method. The monovalent cation ionophore monensin, which inhibits the transport of cellular proteins to the extracellular medium, reduced aggregation of all cells. The data suggest that after initial cell-cell contact continued aggregation is dependent on the secretion of materials to the cell surface. The aggregation of normal and DMD cells was affected similarly by this treatment.

Item Type: Paper
Uncontrolled Keywords: Adolescent Adult Cell Aggregation/*drug effects Child Child, Preschool Culture Techniques Female Fibroblasts/drug effects Furans/*pharmacology Humans Infant Male Middle Aged Monensin/*pharmacology Muscular Dystrophies/*pathology
Subjects: diseases & disorders > congenital hereditary genetic diseases
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > fibroblasts
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > fibroblasts
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > fibroblasts
CSHL Authors:
Communities: Banbury Center
Depositing User: Matt Covey
Date: October 1983
Date Deposited: 17 Jul 2014 20:40
Last Modified: 17 Jul 2014 20:40
URI: http://repository.cshl.edu/id/eprint/30473

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