Tissue culture studies of muscle disorders: Part 2. Biochemical studies, nerve-muscle culture, metabolic myopathies, and animal models

Witkowski, J. A. (May 1986) Tissue culture studies of muscle disorders: Part 2. Biochemical studies, nerve-muscle culture, metabolic myopathies, and animal models. Muscle Nerve, 9 (4). pp. 283-98. ISSN 0148-639X (Print)0148-639x

URL: http://www.ncbi.nlm.nih.gov/pubmed/3520307
DOI: 10.1002/mus.880090402

Abstract

This review continues with studies of protein, lipid, and purine metabolism of Duchenne muscular dystrophy (DMD) cells in vitro and of muscle cells in combined culture with nerve cells. In vitro studies of human metabolic myopathies are tabulated. Results using the hamster, chicken, and mouse (dy25, dy, mdg, and mdx) myopathies are discussed. Interesting findings include suggestions of altered collagen synthesis by DMD cells. Analysis of cell proteins by two-dimensional gel electrophoresis and the use of combined nerve-muscle cultures remain important areas of development. It is disappointing that so few attempts have been made to repeat significant findings in this field, and when a number of laboratories have examined the same phenomenon, the results are often contradictory. It remains to be shown how these various abnormalities found in cells in vitro are related to each other and to those pathologic features of diseased muscle observed in vivo.

Item Type: Paper
Uncontrolled Keywords: Animals Chickens Collagen/metabolism Creatine Kinase/metabolism Cricetinae Culture Techniques Electrophoresis Fibroblasts/metabolism Isoenzymes Lipid Metabolism Lysosomes/enzymology Mice Muscle Proteins/biosynthesis/isolation & purification/metabolism Muscles/*metabolism Muscular Diseases/*metabolism Muscular Dystrophies/etiology/genetics/metabolism Muscular Dystrophy, Animal/metabolism Peptide Hydrolases/metabolism Purines/metabolism Receptors, Cholinergic/analysis Skin
Subjects: Investigative techniques and equipment > cell culture
diseases & disorders > congenital hereditary genetic diseases > muscular dystrophy
CSHL Authors:
Communities: Banbury Center
Depositing User: Matt Covey
Date: May 1986
Date Deposited: 13 Aug 2014 20:01
Last Modified: 13 Aug 2014 20:01
URI: http://repository.cshl.edu/id/eprint/30449

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