Spreading behaviour of cultured fibroblasts from carriers of Duchenne muscular dystrophy

Pizzey, J. A., Witkowski, J. A., Jones, G. E. (February 1987) Spreading behaviour of cultured fibroblasts from carriers of Duchenne muscular dystrophy. Journal of Cell Science, 87 ( P. pp. 163-9. ISSN 0021-9533 (Print)0021-9533

URL: http://www.ncbi.nlm.nih.gov/pubmed/3667711

Abstract

Cultured skin fibroblasts from patients with Duchenne muscular dystrophy (DMD) are more sensitive than normal cells to prolonged exposure to the ionophore monensin. In a cell spreading assay in which cells were preincubated with monensin and subsequently allowed to adhere to and spread on a glass substratum in serum-free medium for 100 min, the mean transformed cell area of normal and DMD cells was 5.97 +/- 0.11 and 5.29 +/- 0.03, respectively. Cultured fibroblasts from carriers of DMD yielded a value of 5.59 +/- 0.03, which is intermediate between, and significantly different from, the values for both normal and DMD cultures. This result would be predicted on the basis of random X-chromosome inactivation in female carriers of this disorder. However, comparison of DMD carrier cell spreading data with data obtained from pooled and summated measurements taken from separate experiments using either normal or DMD fibroblasts suggest a more complex situation. Examination of the variance of the means of cell area for the true carrier population and the summated normal and DMD population provides evidence suggesting that some form of cellular interaction may occur between the two cell genotypes in culture.

Item Type: Paper
Uncontrolled Keywords: Cell Adhesion/drug effects Cells, Cultured Fibroblasts/drug effects/pathology *Heterozygote Humans Monensin/pharmacology Muscular Dystrophies/metabolism/*pathology Skin/*pathology
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell types > fibroblasts
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > fibroblasts
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > fibroblasts

diseases & disorders > congenital hereditary genetic diseases > muscular dystrophy
CSHL Authors:
Communities: Banbury Center
Depositing User: Matt Covey
Date: February 1987
Date Deposited: 01 Dec 2014 15:47
Last Modified: 01 Dec 2014 15:47
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30424

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