Monensin-induced inhibition of cell spreading in normal and dystrophic human fibroblasts

Pizzey, J., Witkowski, J., Jones, G. (August 1984) Monensin-induced inhibition of cell spreading in normal and dystrophic human fibroblasts. Proceedings of the National Academy of Sciences of the United States of America, 81 (15). pp. 4960-4. ISSN 0027-8424

URL: http://www.ncbi.nlm.nih.gov/pubmed/6589639

Abstract

Cultured skin fibroblasts from normal individuals and from patients with Duchenne muscular dystrophy spread equally rapidly when seeded on a glass substratum. Exposure to the ionophore monensin substantially suppresses normal and dystrophic fibroblast spreading in serum-free media for up to at least 100 min. Preincubation of normal fibroblasts with monensin causes a further reduction in cell spreading. Dystrophic fibroblasts fail to spread as well as normal cells after monensin preincubation. Such findings indicate that there is a delay in the secretion of functional adhesive surface proteins in monensin-preincubated normal fibroblasts and that this lag period is significantly longer in dystrophic fibroblasts. These data are consistent with findings of altered adhesive and secretory properties of fibroblasts from patients with Duchenne muscular dystrophy.

Item Type: Paper
Uncontrolled Keywords: Cell Adhesion/*drug effects Cell Movement/drug effects Cells, Cultured Furans/*pharmacology Humans Monensin/*pharmacology Muscular Dystrophies/pathology/*physiopathology
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell types > fibroblasts
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > fibroblasts
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > fibroblasts

diseases & disorders > congenital hereditary genetic diseases > muscular dystrophy
CSHL Authors:
Communities: Banbury Center
Depositing User: Matt Covey
Date: August 1984
Date Deposited: 01 Dec 2014 15:35
Last Modified: 01 Dec 2014 15:35
PMCID: PMC391612
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30423

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