Analysis of skin fibroblast aggregation in Duchenne muscular dystrophy

Jones, G. E., Witkowski, J. A. (April 1981) Analysis of skin fibroblast aggregation in Duchenne muscular dystrophy. Journal of cell science, 48. pp. 291-300. ISSN 0021-9533 (Print)0021-9533

URL: http://www.ncbi.nlm.nih.gov/pubmed/7276091

Abstract

Skin fibroblasts from patients with Duchenne muscular dystrophy have a low intercellular adhesiveness compared with normal cells when aggregated in a Couette viscometer (collision efficiencies of 2.52 and 4.62, respectively). The pattern of aggregation was quantitated using a digitizer system to measure the areas of particles (single cells and aggregates) formed after 20 min aggregation. This size analysis showed that the majority of dystrophic cells remained unaggregated but that a small number of very large aggregates was always formed. Normal cell suspensions only rarely contained large aggregates but contained many intermediate-size aggregates. These differences in intercellular adhesiveness and aggregate pattern formation indicate that there may be an alteration in the surface of dystrophic cells.

Item Type: Paper
Uncontrolled Keywords: Cell Adhesion Cell Aggregation Cells, Cultured Child Child, Preschool Fibroblasts/pathology Humans Infant Muscular Dystrophies/*pathology Skin/*pathology
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell types > fibroblasts
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > fibroblasts
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > fibroblasts

diseases & disorders > congenital hereditary genetic diseases > muscular dystrophy
CSHL Authors:
Communities: Banbury Center
Depositing User: Matt Covey
Date: April 1981
Date Deposited: 26 Nov 2014 17:00
Last Modified: 26 Nov 2014 17:00
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30418

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving