The effect of monensin on cell aggregation of normal and dystrophic human skin fibroblasts

Jones, G. E., Pizzey, J. A., Witkowski, J. A. (August 1985) The effect of monensin on cell aggregation of normal and dystrophic human skin fibroblasts. Experimental Cell Research, 159 (2). pp. 540-5. ISSN 0014-4827

URL: http://www.ncbi.nlm.nih.gov/pubmed/4029279

Abstract

Measurements of aggregation kinetics using couette viscometry show that freshly trypsinized skin fibroblasts from patients with Duchenne muscular dystrophy have values of intercellular adhesiveness approx. 40% those of normal cells. If cells are allowed to recover from the effects of trypsinization (by incubation for 2 h at 37 degrees C in serum-containing medium) the intercellular adhesiveness of both cell types increases, and normal and Duchenne cells aggregate to the same extent. Exposure to the ionophore monensin during the recovery phase leads to suppression of recovery in both cell types, and this effect of the drug is greater in Duchenne fibroblasts. These results are discussed in relation to other data on the reported differential effects of trypsin and monensin on normal and Duchenne fibroblasts.

Item Type: Paper
Uncontrolled Keywords: Cell Aggregation/*drug effects Fibroblasts/drug effects Furans/*pharmacology Humans Monensin/*pharmacology Muscular Dystrophies/*pathology Skin/cytology Trypsin/pharmacology
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell types > fibroblasts
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > fibroblasts
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > fibroblasts

diseases & disorders > congenital hereditary genetic diseases > muscular dystrophy
CSHL Authors:
Communities: Banbury Center
Depositing User: Matt Covey
Date: August 1985
Date Deposited: 21 Nov 2014 21:53
Last Modified: 21 Nov 2014 21:53
URI: http://repository.cshl.edu/id/eprint/30415

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