Maturation of human skeletal muscle fibres in explant tissue culture

Harvey, A. L., Robertson, J. G., Witkowski, J. A. (March 1979) Maturation of human skeletal muscle fibres in explant tissue culture. Journal of the neurological sciences, 41 (1). pp. 115-22. ISSN 0022-510X (Print)0022-510x

URL: http://www.ncbi.nlm.nih.gov/pubmed/438841
DOI: 10.1016/0022-510X(79)90145-X

Abstract

Human foetal skeletal muscle was grown in explant cultures and the development of myotubes was monitored morphologically and by the development of membrane potential and acetycholine sensitivity. Migration of uninucleate cells from the explants occurred in the first day of culture and formation of multinucleated myotubes took place between 7 to 10 days. Early myotubes were variable in appearance, being either flat and nonrefractile or narrow and cylindrical. Some cross-striated cells were observed. Resting membrane potentials were around -25mV and there was great variation in myotube sensitivity to acetylcholine. After about 6 weeks in culture most myotubes were of the refractile type. Many had hypolemmal nuclei and were cross-striated; some contracted spontaneously. All myotubes had developed high sensitivity to acetylcholine although there had been no increase in resting membrane potential.

Item Type: Paper
Uncontrolled Keywords: Acetylcholine/pharmacology *Cell Differentiation Culture Techniques Dose-Response Relationship, Drug Female Gestational Age Humans Membrane Potentials/drug effects Muscle Contraction/drug effects Muscles/*cytology/physiology Pregnancy
Subjects: Investigative techniques and equipment > cell culture
CSHL Authors:
Communities: Banbury Center
Depositing User: Matt Covey
Date: March 1979
Date Deposited: 01 Dec 2014 21:23
Last Modified: 01 Dec 2014 21:23
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30412

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