Expression and activity of the newt Msx-1 gene in relation to limb regeneration

Crews, L., Gates, P. B., Brown, R., Joliot, A., Foley, C., Brockes, J. P., Gann, A. A. (February 1995) Expression and activity of the newt Msx-1 gene in relation to limb regeneration. Proceedings of the Royal Society B: Biological Sciences, 259 (1355). pp. 161-71. ISSN 0962-8452 (Print)0962-8452

URL: http://www.ncbi.nlm.nih.gov/pubmed/7732036
DOI: 10.1098/rspb.1995.0024

Abstract

The Msx-1 homeobox gene is expressed in various contexts during vertebrate development, including the progress zone of the avian and mouse limb bud. Expression of mouse Msx-1 in a cultured myogenic cell line conferred a transformed phenotype and inhibited fusion into myotubes. It has been proposed that Msx-1 expression is required to maintain certain cells in a proliferating and undifferentiated state and may be associated with the ability to regenerate limbs. Urodele amphibians such as the newt regenerate their limbs by formation of a growth zone or blastema, and we have isolated and sequenced newt Msx-1 (NvMsx-1) from a limb blastemal cDNA library. NvMsx-1 expression was detectable in RNA preparations from both limb and tail and their regeneration blastemas, although cultured cells established from limb blastemal mesenchyme gave negative results. When either COS cells or cultured newt blastemal cells were cotransfected with an expression vector for NvMsx-1 and reporter plasmids containing multiple homeobox protein binding sites, NvMsx-1 repressed reporter expression. If NvMsx-1 was expressed together with a marker enzyme in cultured newt blastemal cells, no significant difference in DNA synthesis was observed relative to control transfectants. When myogenic mononucleate cells were transfected with NvMsx-1 and subsequently exposed to low serum to promote fusion, the fraction of Msx-1 positive cells in myotubes was comparable to a control transfected population analysed in the same culture. These results indicate that although Msx-1 expression could be important for limb regeneration, it does not exert a cell-autonomous effect on proliferation or myogenic differentiation of cultured blastemal cells.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Animals Base Sequence Cell Differentiation/genetics Cell Division/genetics Cell Line Cloning, Molecular DNA/genetics Extremities/growth & development/*physiology Gene Expression Regulation, Developmental *Genes, Homeobox Homeodomain Proteins/genetics/physiology MSX1 Transcription Factor Mice Molecular Sequence Data Notophthalmus viridescens/*genetics/growth & development/*physiology Plasmids/genetics Regeneration/*genetics Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Species Specificity *Transcription Factors Transfection
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > homeobox genes
organism description > animal > developmental stage > limb development
CSHL Authors:
Communities: Watson School
Depositing User: Matt Covey
Date: 22 February 1995
Date Deposited: 23 Jun 2014 19:08
Last Modified: 23 Jun 2014 19:08
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30334

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