Phasic, nonsynaptic GABA-A receptor-mediated inhibition entrains thalamocortical oscillations

Rovó, Z., Mátyás, F., Barthó, P., Slézia, A., Lecci, S., Pellegrini, C., Astori, S., Dávid, C., Hangya, B., Lüthi, A., Acsády, L. (2014) Phasic, nonsynaptic GABA-A receptor-mediated inhibition entrains thalamocortical oscillations. Journal of Neuroscience, 34 (21). pp. 7137-7147. ISSN 15292401

[img] PDF (Paper)
Hangya J Neuroscience 2014.pdf - Published Version
Restricted to Repository staff only until 1 December 2014.

Download (2656Kb)
URL: http://www.ncbi.nlm.nih.gov/pubmed/24849349
DOI: 10.1523/JNEUROSCI.4386-13.2014

Abstract

GABA-A receptors (GABA-ARs) are typically expressed at synaptic or nonsynaptic sites mediating phasic and tonic inhibition, respectively. These two forms of inhibition conjointly control various network oscillations. To disentangle their roles in thalamocortical rhythms, we focally deleted synaptic, γ2 subunit-containing GABA-ARs in the thalamus using viral intervention in mice. After successful removal of γ2 subunit clusters, spontaneous and evoked GABAergic synaptic currents disappeared in thalamocortical cells when the presynaptic, reticular thalamic (nRT) neurons fired in tonic mode. However, when nRT cells fired in burst mode, slow phasic GABA-AR-mediated events persisted, indicating a dynamic, burst-specific recruitment of nonsynaptic GABA-ARs. In vivo, removal of synaptic GABA-ARs reduced the firing of individual thalamocortical cells but did not abolish slow oscillations or sleep spindles. We conclude that nonsynaptic GABA-ARs are recruited in a phasic manner specifically during burst firing of nRT cells and provide sufficient GABA-AR activation to control major thalamocortical oscillations.

Item Type: Paper
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell types > interneurons > GABAergic interneurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > interneurons > GABAergic interneurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > interneurons > GABAergic interneurons
CSHL Authors:
Communities: CSHL labs > Kepecs lab
Depositing User: Matt Covey
Date Deposited: 09 Jun 2014 20:20
Last Modified: 09 Jun 2014 20:20
PMCID: PMC4028493
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30279

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving