Preparation of plasma membrane from isolated neurons

Henn, F. A., Hansson, H. A., Hamberger, A. (1972) Preparation of plasma membrane from isolated neurons. Journal of Cell Biology, 53 (3). pp. 654-61. ISSN 0021-9525

URL: http://www.ncbi.nlm.nih.gov/pubmed/4402165
DOI: 10.1083/jcb.53.3.654

Abstract

A bulk fraction enriched with respect to neuronal cell bodies was used as starting material for the isolation of neuronal plasma membrane The cells were gently homogenized in isotonic sucrose and a crude membrane containing fraction sedimented at 3000 g. Subsequently, the membrane fraction was purified on a discontinuous sucrose density gradient between 35% and 25 5% sucrose (w/w). Enzymatic analyses showed a 4-5-fold enrichment in plasma membrane markers, and a 10-15% contamination of mitochondrial and microsomal material. Electron micrographs of the membrane fraction confirmed the enzymatic data Fragmented membranes were found, mainly in vesicular form No ribosomes, but a few mitochondria and some multilamellar membranes were seen

Item Type: Paper
Uncontrolled Keywords: Adenosine Triphosphatases/analysis Animals Cell Fractionation *Cell Membrane/enzymology Cell Nucleolus Cell Nucleus Centrifugation, Density Gradient Cerebral Cortex/cytology/enzymology Cytochromes Electron Transport Complex IV/analysis Glucosephosphates/analysis Methods Microscopy, Electron Microsomes/enzymology Mitochondria/enzymology Monoamine Oxidase/analysis Nadp Neurons/*cytology/enzymology Oxidoreductases/analysis Rabbits Sucrose
Subjects: organs, tissues, organelles, cell types and functions > tissues types and functions > membranes
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
CSHL Authors:
Communities: CSHL labs > Henn lab
Depositing User: Matt Covey
Date Deposited: 09 Jul 2014 18:44
Last Modified: 09 Jul 2014 18:44
PMCID: PMC2108764
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30201

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