Fluctuations in intracellular calcium responses to action potentials in single en passage presynaptic boutons of layer V neurons in neocortical slices

Frenguelli, Bruno G., Malinow, Roberto (September 1996) Fluctuations in intracellular calcium responses to action potentials in single en passage presynaptic boutons of layer V neurons in neocortical slices. Learning and Memory, 3 (2-3). pp. 150-9. ISSN 1072-0502 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/10456085
DOI: 10.1101/lm.3.2-3.150

Abstract

The release of neurotransmitter from a nerve terminal on invasion by an action potential shows large trial-to-trial fluctuations. The factors contributing to this variability have not been elucidated clearly. Here, simultaneous patch-clamp and optical measurements from layer V neocortical pyramidal neurons have been used to assess the extent to which the fluctuations in transmitter release may be caused by variability in calcium rise in presynaptic structures. Boutons on axon collaterals were visualized and increases in intracellular calcium, assessed by Fura-2, were observed in response to single action potentials. In some boutons, calcium responses showed trial-to-trial variability and occasional apparent failures despite the faithful conduction of the action potential. These results suggest that a factor contributing to the fluctuation in transmitter release may be the variability with which depolarization of a presynaptic bouton produces an increase in intrabouton calcium.

Item Type: Paper
Uncontrolled Keywords: Action Potentials/physiology Animals Calcium/ metabolism Electrophysiology Immunohistochemistry In Vitro Intracellular Membranes/ metabolism Microscopy, Fluorescence Neocortex/ physiology Neurons/ physiology/ultrastructure Presynaptic Terminals/ physiology Rats Research Support, Non-U.S. Gov't
Subjects: neurobiology
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > pyramidal neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > pyramidal neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > pyramidal neurons

organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > synapse
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > synaptic transmission
CSHL Authors:
Communities: CSHL labs > Malinow lab
Depositing User: Matt Covey
Date: September 1996
Date Deposited: 22 May 2014 14:34
Last Modified: 22 May 2014 14:34
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30180

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