RAC regulation of actin polymerization and proliferation by a pathway distinct from Jun kinase

Joneson, T., McDonough, M., Bar-Sagi, D., Van Aelst, L. (November 1996) RAC regulation of actin polymerization and proliferation by a pathway distinct from Jun kinase. Science, 274 (5291). pp. 1374-1376. ISSN 0036-8075

URL: http://www.ncbi.nlm.nih.gov/pubmed/8910277
DOI: 10.1126/science.274.5291.1374

Abstract

The RAC guanine nucleotide binding proteins regulate multiple biological activities, including actin polymerization, activation of the Jun kinase (JNK) cascade, and cell proliferation. RAC effector loop mutants were identified that separate the ability of RAC to interact with different downstream effecters. One mutant of activated human RAC protein, RAC(V12H40) (with valine and histidine substituted at position 12 and 40, respectively), was defective in binding to PAK3, a Ste20-related p21-activated kinase (PAK), but bound to PORI, a RAG-binding protein. This mutant failed to stimulate PAK and JNK activity but still induced membrane ruffling and mediated transformation. A second mutant, RAC(V12L37) (with leucine substituted at position 37), which bound PAK but not PORI, induced JNK activation but was defective in inducing membrane ruffling and transformation, These results indicate that the effects of RAC on the JNK cascade and on actin polymerization and cell proliferation are mediated by distinct effector pathways that diverge at the level of RAC itself.

Item Type: Paper
Uncontrolled Keywords: ACTIVATED PROTEIN-KINASES BINDING GTPASES TRANSFORMATION CDC42 RHOA PHOSPHORYLATION IDENTIFICATION DOMAIN
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > actin
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase
CSHL Authors:
Communities: CSHL labs > Van Aelst lab
Depositing User: Matt Covey
Date: November 1996
Date Deposited: 22 May 2014 15:33
Last Modified: 22 May 2014 15:33
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30166

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