Telomerase regulation during entry into the cell cycle in normal human T cells

Buchkovich, K. J., Greider, C. W. (September 1996) Telomerase regulation during entry into the cell cycle in normal human T cells. Molecular Biology of the Cell, 7 (9). pp. 1443-54. ISSN 1059-1524 (Print)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/8885238
DOI: 10.1091/mbc.7.9.1443

Abstract

Telomerase activity is involved in telomere length maintenance. Leukocytes, unlike many human somatic tissues, have detectable telomerase activity. These cells provide a normal human cell type in which to study telomerase. We studied the regulation of telomerase activity and the telomerase RNA component as leukocytes were stimulated to enter the cell cycle. In primary human leukocytes stimulated with phytohemagglutinin, telomerase activity increased > 10-fold as naturally quiescent cells entered the cell cycle. Antibodies to the T cell receptor (TCR)/CD3 complex and the costimulatory CD28 receptor induced telomerase activity in a T cell-enriched population of cells. Rapamycin, an immunosuppressant that blocks TCR/CD3 signal transduction pathways and cdk2 activation, blocked telomerase induction. Hydroxyurea, an inhibitor of S phase, did not block cdk2 kinase activity or telomerase activation. In summary, telomerase is regulated in G1 phase as normal human T cells enter the cell cycle.

Item Type: Paper
Uncontrolled Keywords: Antibodies/pharmacology Antigens, CD2/analysis Antigens, CD20/analysis Antigens, CD28/metabolism B-Lymphocytes/cytology Blotting, Northern CDC2-CDC28 Kinases Cell Cycle/drug effects/ physiology Cell Cycle Proteins Cells, Cultured Cyclin-Dependent Kinase 2 Cyclin-Dependent Kinase Inhibitor p27 Cyclin-Dependent Kinases/drug effects/metabolism Cyclins/biosynthesis/drug effects Enzyme Inhibitors/pharmacology Humans Hydroxyurea/pharmacology Immunosuppressive Agents/pharmacology Interleukin-2/pharmacology Leukocytes/cytology Microtubule-Associated Proteins/biosynthesis/drug effects Phytohemagglutinins/pharmacology Polyenes/pharmacology Polymerase Chain Reaction/methods Protein-Serine-Threonine Kinases/drug effects/metabolism RNA/metabolism Receptor-CD3 Complex, Antigen, T-Cell/immunology/metabolism Research Support, U.S. Gov't, P.H.S. Sirolimus T-Lymphocytes/drug effects/ physiology Telomerase/drug effects/genetics/ metabolism Tumor Suppressor Proteins Up-Regulation
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell types > T cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > T cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > T cells

organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > telomerase
CSHL Authors:
Communities: CSHL labs > Greider lab
Depositing User: Matt Covey
Date: September 1996
Date Deposited: 22 May 2014 15:10
Last Modified: 22 May 2014 15:10
PMCID: PMC275993
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30165

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