Differential control of transcription by homologous homeodomain coregulators

Huang, C. C., Herr, W. (1996) Differential control of transcription by homologous homeodomain coregulators. Mol Cell Biol, 16 (6). pp. 2967-76. ISSN 0270-7306 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/8649408

Abstract

The human herpes simplex virus type 1 (HSV-1) transactivator VP16 and its homolog from bovine herpes-virus 1 (BHV-1) can each recruit the human homeodomain protein Oct-1 into a transcriptional regulatory complex. Here, we show that these two Oct-1 coregulators possess similar, if not identical, homeodomain recognition properties but possess different virus-specific cis-regulatory specificities: the HSV-1 VP-16 protein activates transcription from the HSV-1 VP16 response element, and the BHV-1 VP16 protein activates transcription from the BHV-1 VP16 response element. A distinct 3-bp segment, the D segment, lying 3' of the canonical TAATGARAT motif (where R is a purine) in the VP16 response element is responsible for the differential cis element recognition and transcriptional activation by these two homeodomain coregulators. These results demonstrate how a single homeodomain protein can direct differential transcriptional regulation by selective association with homologous homeodomain coregulators.

Item Type: Paper
Uncontrolled Keywords: Animals Base Sequence Binding Sites/genetics Cattle Consensus Sequence DNA, Viral/genetics/metabolism DNA-Binding Proteins/genetics/metabolism Genes, Regulator Genes, Viral Hela Cells Herpesvirus 1, Bovine/ genetics Herpesvirus 1, Human/ genetics Homeodomain Proteins/ genetics/metabolism Host Cell Factor C1 Humans Molecular Sequence Data Octamer Transcription Factor-1 Octamer Transcription Factor-2 Research Support, U.S. Gov't, P.H.S. Trans-Activators/ genetics/metabolism Transcription Factors/ genetics/metabolism Transcription, Genetic
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > homeodomain protein
CSHL Authors:
Communities: CSHL labs > Herr lab
Depositing User: Matt Covey
Date Deposited: 22 May 2014 16:29
Last Modified: 22 May 2014 16:29
PMCID: PMC231291
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30152

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