Maturation of a central glutamatergic synapse

Wu, G.-Y., Malinow, R., Cline, H. T. (1996) Maturation of a central glutamatergic synapse. Science, 274 (5289). pp. 972-6. ISSN 0036-8075 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/8875937
DOI: 10.1126/science.274.5289.972

Abstract

Whole-cell recordings from optic tectal neurons in Xenopus tadpoles were used to study the maturation of a glutamatergic synapse. The first glutamatergic transmission is mediated only by N-methyl-D-aspartate (NMDA) receptors and is silent at resting potentials. More mature synapses acquire transmission by alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptors. This maturational program is mimicked by postsynaptic expression of constitutively active calcium-calmodulin-dependent protein kinase II (CaMKII). Newly formed synapses may be silent unless sufficient depolarization is provided by coincident activity that could activate postsynaptic CaMKII, resulting in the appearance of AMPA responses.

Item Type: Paper
Uncontrolled Keywords: Animals Ca(2+)-Calmodulin Dependent Protein Kinase/genetics/ metabolism Evoked Potentials Glutamates/ physiology In Vitro N-Methylaspartate/metabolism Neurons/cytology/enzymology/physiology/virology Patch-Clamp Techniques Receptors, AMPA/ physiology Receptors, N-Methyl-D-Aspartate/ physiology Recombinant Proteins/metabolism Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Superior Colliculus/cytology/physiology Synapses/ physiology Synaptic Transmission Xenopus alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid/metabolism
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > AMPA receptor
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > NMDA receptor
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase > CaMKII
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons

organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > synapse
organism description > animal > Frog > xenopus
CSHL Authors:
Communities: CSHL labs > Cline lab
CSHL labs > Malinow lab
Depositing User: Kathleen Darby
Date Deposited: 12 May 2014 17:05
Last Modified: 12 May 2014 17:05
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30125

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