Direct measurement of coupling between dendritic spines and shafts

Svoboda, K., Tank, D. W., Denk, W. (May 1996) Direct measurement of coupling between dendritic spines and shafts. Science, 272 (5262). pp. 716-9. ISSN 0036-8075

URL: http://www.ncbi.nlm.nih.gov/pubmed/8614831
DOI: 10.1126/science.272.5262.716

Abstract

Characterization of the diffusional and electrotonic coupling of spines to the dendritic shaft is crucial to understanding neuronal integration and synaptic plasticity. Two-photon photobleaching and photorelease of fluorescein dextran were used to generate concentration gradients between spines and shafts in rat CA1 pyramidal neurons. Diffusional reequilibration was monitored with two-photon fluorescence imaging. The time course of reequilibration was exponential, with time constants in the range of 20 to 100 milliseconds, demonstrating chemical compartmentalization on such time scales. These values imply that electrical spine neck resistances are unlikely to exceed 150 megohms and more likely range from 4 to 50 megohms.

Item Type: Paper
Uncontrolled Keywords: Animals Dendrites/metabolism/*physiology/ultrastructure Dextrans/metabolism Diffusion Electric Conductivity Electric Impedance Fluoresceins/metabolism Fluorescence Kinetics Microscopy/methods Models, Neurological Neuronal Plasticity Pyramidal Cells/metabolism/*physiology/ultrastructure Rats
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic spines
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic spines
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic spines

Investigative techniques and equipment > microscopy
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > pyramidal neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > pyramidal neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > pyramidal neurons
CSHL Authors:
Communities: CSHL labs > Svoboda lab
Depositing User: Kathleen Darby
Date: 3 May 1996
Date Deposited: 13 May 2014 14:04
Last Modified: 13 May 2014 14:04
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30113

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