Examination of the role of cGMP in long-term potentiation in the CA1 region of the hippocampus

Selig, D. K., Segal, M. R., Liao, D., Malenka, R. C., Malinow, R., Nicoll, R. A., Lisman, J. E. (July 1996) Examination of the role of cGMP in long-term potentiation in the CA1 region of the hippocampus. Learning and Memory, 3 (1). pp. 42-8. ISSN 1072-0502 (Print)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/10456075
DOI: 10.1101/lm.3.1.42

Abstract

The mechanisms underlying the generation of NMDA receptor-dependent LTP in the CA1 region of the hippocampus continue to receive a great deal of attention because of the postulated importance of LTP as a synaptic mechanism for learning and memory. It is well accepted that the initial induction of LTP occurs in the postsynaptic cell, but the site of expression remains controversial. One prominent hypothesis is that LTP involves the release of one or more retrograde messengers that act on the presynaptic terminal to enhance transmitter release. Recently, evidence has been presented that retrograde messengers function to activate presynaptic guanylyl cyclase and that the resulting rise in presynaptic cGMP levels, when accompanied by presynaptic activity, is responsible for generating an early component of LTP. We have tested this hypothesis by examining whether synaptic strength is increased by coupling tetanic stimulation with application of a membrane-permeable analog of cGMP. The experiments were done in the presence of an NMDA receptor antagonist to block postsynaptic induction mechanisms. Under a variety of experimental conditions, this manipulation failed to generate LTP, suggesting that an increase in cGMP levels accompanied by presynaptic activity is not sufficient to generate LTP in the CA1 region of the hippocampus.

Item Type: Paper
Additional Information: 1072-0502 (Print) In Vitro Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.
Uncontrolled Keywords: 2-Amino-5-phosphonovalerate/pharmacology Animals Cyclic GMP/analogs & derivatives/pharmacology/*physiology Electric Stimulation Excitatory Amino Acid Antagonists/pharmacology Female Guinea Pigs Hippocampus/drug effects/*physiology Long-Term Potentiation/*physiology Osmolar Concentration Rats Synapses/drug effects/physiology
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > Long term potentiation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > NMDA receptor
organs, tissues, organelles, cell types and functions > tissues types and functions > hippocampus
organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > synapse
CSHL Authors:
Communities: CSHL labs > Malinow lab
Depositing User: Kathleen Darby
Date: July 1996
Date Deposited: 13 May 2014 16:11
Last Modified: 13 May 2014 16:11
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30107

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