Linkage of replication to start by the Cdk inhibitor Sic1

Schneider, B. L., Yang, Q. H., Futcher, A. B. (1996) Linkage of replication to start by the Cdk inhibitor Sic1. Science, 272 (5261). pp. 560-2. ISSN 0036-8075 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/8614808
DOI: 10.1126/science.272.5261.560

Abstract

In Saccharomyces cerevisiae, three G1 cyclins (Clns) are important for Start, the event committing cells to division. Sic1, an inhibitor of C1b-Cdc28 kinases, became phosphorylated at Start, and this phosphorylation depended on the activity of Clns. Sic1 was subsequently lost, which depended on the activity of Clns and the ubiquitin-conjugating enzyme Cdc34. Inactivation of Sic1 was the only nonredundant essential function of Clns, because a sic1 deletion rescued the inviability of the cln1 cln2 cln3 triple mutant. In sic1 mutants, DNA replication became uncoupled from budding. Thus, Sic1 may be a substrate of Cln-Cdc28 complexes, and phosphorylation and proteolysis of Sic1 may regulate commitment to replication at Start.

Item Type: Paper
Uncontrolled Keywords: CDC28 Protein Kinase, S cerevisiae/antagonists & inhibitors/metabolism Cell Cycle Cyclins/metabolism DNA Replication DNA, Fungal/biosynthesis Enzyme Inhibitors/ metabolism Fungal Proteins/ metabolism Ligases/metabolism Phosphorylation Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Saccharomyces cerevisiae/cytology/ metabolism Saccharomyces cerevisiae Proteins Ubiquitin-Protein Ligase Complexes Ubiquitin-Protein Ligases
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
organism description > yeast > Saccharomyces
organs, tissues, organelles, cell types and functions > organelles, types and functions > anaphase
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression > phosphorylation
CSHL Authors:
Communities: CSHL labs > Futcher lab
Depositing User: Kathleen Darby
Date Deposited: 13 May 2014 16:03
Last Modified: 13 May 2014 16:03
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30106

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