Effects of pancreatic spasmolytic Polypeptide (PSP) on epithelial cell function

Otto, W. R., Rao, J., Cox, H. M., Kotzian, E., Lee, C. Y., Goodlad, R. A., Lane, A., Gorman, M., Freemont, P. A., Hansen, H. F., Pappin, D., Wright, N. A. (January 1996) Effects of pancreatic spasmolytic Polypeptide (PSP) on epithelial cell function. European Journal of Biochemistry, 235 (1-2). pp. 64-72. ISSN 0014-2956 (Print)0014-2956 (Linking)

Abstract

Trefoil peptides are expressed near endodermal ulcerations and may modulate epithelial repair. The trefoil pancreatic spasmolytic polypeptide (PSP) was tested for growth activity in vitro on epithelial cells and in vivo following intragastric or intravenous infusion in parenterally fed intact rats. Ion transport was assessed as changes in short-circuit current in rat intestine and adenocarcinoma cells in Ussing chambers. PSP stimulated growth of MCF-7 and Colo-357 cells, but only in the presence of extracellular glutathione (GSH). The effect was attenuated by GSH depletion with buthionine sulphoximine, even in GSH-containing media. When GSH-reduced PSP was carboxymethylated with iodoacetic acid, it still depended on extracellular GSH for its growth effect. Intestinal epithelial proliferation in rats was not affected by either intravenous or intraluminal infusion. PSP had no effect on basal or stimulated ion flux in rat jejunum or epithelial monolayers. The peptide did not compete with 125I-labeled epidermal growth factor for its receptor. [14C]Iodoacetamide treatment of PSP, followed by prolonged tryptic digestion yielded predominantly a 14C-labeled tetrapeptide fragment containing Cys1O4, with a lesser quantity of a 14C-labeled 15-amino-acid peptide containing Cys95 (molar ratio 15:1). GSH may predominantly reduce the Cys6-Cys1O4 terminal disulphide bond in PSP. We conclude that some epithelia may exhibit a growth response to PSP if extracellular GSH is present. Reduction of PSP by GSH is not necessary for this response, suggesting that the trefoil receptor or its signal transduction is GSH sensitive. PSP could assist wound healing by interactions with epithelial cells exposed concurrently to a local high GSH concentration.

Item Type:	Paper
Additional Information:	Otto, W R Rao, J Cox, H M Kotzian, E Lee, C Y Goodlad, R A Lane, A Gorman, M Freemont, P A Hansen, H F Pappin, D Wright, N A Wellcome Trust/United Kingdom Research Support, Non-U.S. Gov't Germany European journal of biochemistry / FEBS Eur J Biochem. 1996 Jan 15;235(1-2):64-72.
Uncontrolled Keywords:	Animals Cell Division/drug effects Cell Line Digestive System/cytology/drug effects Epidermal Growth Factor/metabolism Epithelial Cells Epithelium/drug effects/physiology Glutathione/pharmacology Ion Transport/drug effects Jejunum/drug effects/metabolism Male Mucins Muscle Proteins Neuropeptides Oxidation-Reduction Peptides/chemistry/ pharmacology Rats Rats, Sprague-Dawley Rats, Wistar
Subjects:	organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell division organs, tissues, organelles, cell types and functions > cell types and functions > cell types > cell line organs, tissues, organelles, cell types and functions > cell types and functions > cell types > cell line organs, tissues, organelles, cell types and functions > cell types and functions > cell types > cell line organs, tissues, organelles, cell types and functions > cell types and functions > cell types > epithelial cell organs, tissues, organelles, cell types and functions > cell types and functions > cell types > epithelial cell organs, tissues, organelles, cell types and functions > cell types and functions > cell types > epithelial cell organs, tissues, organelles, cell types and functions > tissues types and functions > epithelium organs, tissues, organelles, cell types and functions > tissues types and functions > ion transport organs, tissues, organelles, cell types and functions > tissues types and functions > transport > ion transport
CSHL Authors:	Pappin, Darryl J.
Communities:	CSHL labs > Pappin lab
Depositing User:	Kathleen Darby
Date:	15 January 1996
Date Deposited:	13 May 2014 15:15
Last Modified:	13 May 2014 15:15
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/30099

Actions (login required)

Administrator's edit/view item