p53-dependent association between cyclin G and the B' subunit of protein phosphatase 2A

Okamoto, K., Kamibayashi, C., Serrano, M., Prives, C., Mumby, M. C., Beach, D. (1996) p53-dependent association between cyclin G and the B' subunit of protein phosphatase 2A. Molecular and Cellular Biology, 16 (11). pp. 6593-602. ISSN 0270-7306

URL: http://www.ncbi.nlm.nih.gov/pubmed/8887688

Abstract

We and others previously showed that cyclin G is a transcriptional target of the p53 tumor suppressor protein. However, cellular proteins which might form a complex with cyclin G have not yet been identified. To gain insight into the biological role of cyclin G, we used the yeast two-hybrid screen and isolated two mouse cDNAs encoding cyclin G-interacting proteins. Interestingly, both positive cDNAs encoded B' regulatory subunits of protein phosphatase 2A (PP2A). One clone encodes B'alpha, while the other clone codes for a new member of the B' family, B'beta. B'beta is 70% identical to other members of the B' family. B'alpha associated both in vitro and in vivo with cyclin G but not with the other mammalian cyclins. Furthermore, cyclin G formed a complex with B'alpha only after induction of p53 in p53 temperature-sensitive cell lines. These results indicate that cyclin G forms a specific complex with the B' subunit of PP2A and that complex formation is regulated by p53. Potential roles for the cyclin G-B' complex in p53-mediated pathways are discussed.

Item Type: Paper
Uncontrolled Keywords: Alternative Splicing Amino Acid Sequence Animals Baculoviridae COS Cells Cell Line Cloning, Molecular Cyclins/chemistry/ metabolism Macromolecular Substances Mammals Mice Molecular Sequence Data Open Reading Frames Protein-Tyrosine-Phosphatase/chemistry/ metabolism Recombinant Proteins/chemistry/metabolism Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Saccharomyces cerevisiae/growth & development Sequence Homology, Amino Acid Spodoptera Transfection Tumor Suppressor Protein p53/ metabolism
Subjects: organism description > animal > insect > Spodoptera
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > cell line
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > cell line
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > cell line

Investigative techniques and equipment > cloning
Investigative techniques and equipment > assays > cloning

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > p53
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > protein phosphatase
Investigative techniques and equipment > transfection
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > tumor suppressor
CSHL Authors:
Communities: CSHL labs > Beach lab
Depositing User: Kathleen Darby
Date Deposited: 13 May 2014 15:11
Last Modified: 13 May 2014 15:11
PMCID: PMC231661
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30098

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