A connection between pre-mRNA splicing and the cell cycle in fission yeast: cdc28+ is allelic with prp8+ and encodes an RNA-dependent ATPase/helicase

Lundgren, K., Allan, S., Urushiyama, S., Tani, T., Ohshima, Y., Frendewey, D., Beach, D. (1996) A connection between pre-mRNA splicing and the cell cycle in fission yeast: cdc28+ is allelic with prp8+ and encodes an RNA-dependent ATPase/helicase. Molecular Biology of the Cell, 7 (7). pp. 1083-94. ISSN 1059-1524

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URL: http://www.ncbi.nlm.nih.gov/pubmed/8862522
DOI: 10.1091/mbc.7.7.1083

Abstract

The fission-yeast gene cdc28+ was originally identified in a screen for temperature-sensitive mutants that exhibit a cell-division cycle arrest and was found to be required for mitosis. We undertook a study of this gene to understand more fully the general requirements for entry into mitosis. Cells carrying the conditional lethal cdc28-P8 mutation divide once and arrest in G2 after being shifted to the restrictive temperature. We cloned the cdc28+ gene by complementation of the temperature-sensitive growth arrest in cdc28-P8. DNA sequence analysis indicated that cdc28+ encodes a member of the DEAH-box family of putative RNA-dependent ATPases or helicases. The Cdc28 protein is most similar to the Prp2, Prp16, and Prp22 proteins from budding yeast, which are required for the splicing of mRNA precursors. Consistent with this similarity, the cdc28-P8 mutant accumulates unspliced precursors at the restrictive temperature. Independently, we isolated a temperature-sensitive pre-mRNA splicing mutant prp8-1 that exhibits a cell-cycle phenotype identical to that of cdc28-P8. We have shown that cdc28 and prp8 are allelic. These results suggest a connection between pre-mRNA splicing and progression through the cell cycle.

Item Type: Paper
Uncontrolled Keywords: Adenosinetriphosphatase/genetics/metabolism Alleles Amino Acid Sequence Base Sequence CDC28 Protein Kinase, S cerevisiae/genetics/metabolism Cell Cycle/genetics/physiology Cloning, Molecular DNA, Fungal/genetics Fungal Proteins/genetics/metabolism Genes, Fungal Molecular Sequence Data Phenotype RNA Helicases RNA Nucleotidyltransferases/genetics/metabolism RNA Precursors/genetics/ metabolism RNA Splicing RNA, Fungal/genetics/ metabolism Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Restriction Mapping Saccharomyces cerevisiae Proteins Schizosaccharomyces/cytology/ genetics/ metabolism Sequence Homology, Amino Acid
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > ATPase
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle
Investigative techniques and equipment > cloning
Investigative techniques and equipment > assays > cloning

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > helicase
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > nuclear ribonucleoprotein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > pre-mRNA
organism description > yeast > Saccharomyces > Schizosaccharomyces pombe
organism description > yeast
CSHL Authors:
Communities: CSHL labs > Beach lab
Depositing User: Kathleen Darby
Date Deposited: 13 May 2014 16:27
Last Modified: 13 May 2014 16:27
PMCID: PMC275960
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30084

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