Fluorescent protein marker lines in maize: generation and applications

Wu, Q., Luo, A., Zadrozny, T., Sylvester, A., Jackson, D. (2013) Fluorescent protein marker lines in maize: generation and applications. The International Journal of Developmental Biology, 57 (6-8). pp. 535-43. ISSN 0214-6282

URL: http://www.ncbi.nlm.nih.gov/pubmed/24166436
DOI: 10.1387/ijdb.130240qw

Abstract

Fluorescent proteins (FP) have significantly impacted the way that we study plants in the past two decades. In the post-genomics era, these FP tools are in higher demand by plant scientists for studying the dynamics of protein localization, function, and interactions, and to translate sequence information to biological knowledge that can benefit humans. Although FP tools have been widely used in the model plant Arabidopsis, few FP resources have been developed for maize, one of the most important food crops worldwide, and an ideal species for genetic and developmental biology research. In an effort to provide the maize and cereals research communities with a comprehensive set of FP resources for different purposes of study, we generated more than 100 stable transformed maize FP marker lines, which mark most compartments in maize cells with different FPs. Additionally, we are generating driver and reporter lines, based on the principle of the pOp-LhG4 transactivation system, allowing specific expression or mis-expression of any gene of interest to precisely study protein functions. These marker lines can be used not only for static protein localization studies, but will be useful for studying protein dynamics and interactions using kinetic microscopy methods, such as fluorescence recovery after photobleaching (FRAP), fluorescence correlation spectroscopy (FCS), and fluorescence resonance energy transfer (FRET).

Item Type: Paper
Subjects: organism description > plant > maize
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > green fluorescent protein
organism description > plant
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > transgenic plants
CSHL Authors:
Communities: CSHL labs > Jackson lab
Depositing User: Matt Covey
Date: 2013
Date Deposited: 12 May 2014 15:34
Last Modified: 12 May 2014 15:34
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30049

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