The chaperonin CCT8 facilitates spread of tobamovirus infection

Fichtenbauer, D., Xu, X. M., Jackson, D., Kragler, F. (March 2012) The chaperonin CCT8 facilitates spread of tobamovirus infection. Plant Signaling & Behavior, 7 (3). pp. 318-21. ISSN 1559-2316

URL: http://www.ncbi.nlm.nih.gov/pubmed/22476462
DOI: 10.4161/psb.19152

Abstract

The homeodomain transcription factor KNOTTED1 (KN1) functions in shoot meristem maintenance and is thought to move from cell to cell in a similar fashion as viral movement proteins. Both types of transported proteins bind to RNA, and associate with intercellular bridges formed by plasmodesmata. In a mutant screen for KN1 transport deficiency, a component of a type II chaperonin complex, CCT8, was identified, and found to interact with non-cell-autonomous proteins. The cct8 mutants are characterized by limited functionality of non-cell-autonomous proteins after their movement, and a phenotype resembling lack of homeodomain protein activity. Evidence suggests that CCT8 functions in post-translocational refolding of transported proteins. Here we show that spread of tobamovirus infection is reduced in a cct8 mutant. This suggests that similar to KN1, viral movement proteins are unfolded and refolded during transport to gain functionality in the receiving cells.

Item Type: Paper
Uncontrolled Keywords: Chaperonins/genetics/*metabolism Meristem Plant Diseases/*virology Plant Proteins/genetics/*metabolism Tobamovirus/*pathogenicity
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > chaperonins
organism description > plant
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
organism description > virus
CSHL Authors:
Communities: CSHL labs > Jackson lab
Depositing User: Matt Covey
Date: March 2012
Date Deposited: 12 May 2014 15:02
Last Modified: 12 May 2014 15:02
PMCID: PMC3443910
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30048

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving